CKIA系统评价icsi选择的无精子障碍男性附睾精子样本

Liliana Ramos, Peter de Boer, Eric J. H. Meuleman, Didi D. M. Braat, Alex M. M. Wetzels
{"title":"CKIA系统评价icsi选择的无精子障碍男性附睾精子样本","authors":"Liliana Ramos,&nbsp;Peter de Boer,&nbsp;Eric J. H. Meuleman,&nbsp;Didi D. M. Braat,&nbsp;Alex M. M. Wetzels","doi":"10.1002/j.1939-4640.2004.tb02807.x","DOIUrl":null,"url":null,"abstract":"<p><b>ABSTRACT: </b> The objective of this study was to evaluate nuclear normality in intracytoplasmic sperm injection (ICSI)-selected epididymal sperm from obstructive azoospermic (OA) patients. We evaluated whether the selection criteria used in routine ICSI (morphology and motility at a magnification of 400×) is adequate for selecting “normal” sperm from epididymal samples. Surgically retrieved spermatozoa from the caput epididymis of 15 OA patients and ejaculated sperm samples from 9 normospermic donors were evaluated with a DNA-specific stain (Feulgen) and in combination with the computerized karyometric image analysis (CKIA) system. Original (unselected) samples and ICSI-selected sperm were compared in donor and patient samples. In the original fraction, a larger variation in almost all measured parameters was found in epididymal sperm than in ejaculated sperm. After sperm selection, the morphometry was comparable between epididymal and ejaculated sperm. However, for those parameters related to the DNA stainability and chromatin texture (nuclear condensation), significant differences between patients and donors were observed. This result suggests that the size and form of the sperm do not necessarily hold similar internal structures. Thus, the frequency of “normal” sperm significantly increased after ICSI selection, but the improvement was more marked in donor than in OA patients' samples. In conclusion, at least a twofold increase in the number of normal spermatozoa was achieved after ICSI selection. The heterogeneity in the stainability and chromatin condensation of epididymal samples from OA patients indicates that some of the selected spermatozoa have a hypocondensed or hypercondensed chromatin. Even in the best of donor cases, no more than 55% of the selected sperm scored normal with CKIA, indicating that the present routine ICSI selection criteria are not sufficient for selecting normal condensed nuclei.</p>","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"25 3","pages":"406-411"},"PeriodicalIF":0.0000,"publicationDate":"2013-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/j.1939-4640.2004.tb02807.x","citationCount":"26","resultStr":"{\"title\":\"Evaluation of ICSI-Selected Epididymal Sperm Samples of Obstructive Azoospermic Males by the CKIA System\",\"authors\":\"Liliana Ramos,&nbsp;Peter de Boer,&nbsp;Eric J. H. Meuleman,&nbsp;Didi D. M. Braat,&nbsp;Alex M. M. Wetzels\",\"doi\":\"10.1002/j.1939-4640.2004.tb02807.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><b>ABSTRACT: </b> The objective of this study was to evaluate nuclear normality in intracytoplasmic sperm injection (ICSI)-selected epididymal sperm from obstructive azoospermic (OA) patients. We evaluated whether the selection criteria used in routine ICSI (morphology and motility at a magnification of 400×) is adequate for selecting “normal” sperm from epididymal samples. Surgically retrieved spermatozoa from the caput epididymis of 15 OA patients and ejaculated sperm samples from 9 normospermic donors were evaluated with a DNA-specific stain (Feulgen) and in combination with the computerized karyometric image analysis (CKIA) system. Original (unselected) samples and ICSI-selected sperm were compared in donor and patient samples. In the original fraction, a larger variation in almost all measured parameters was found in epididymal sperm than in ejaculated sperm. After sperm selection, the morphometry was comparable between epididymal and ejaculated sperm. However, for those parameters related to the DNA stainability and chromatin texture (nuclear condensation), significant differences between patients and donors were observed. This result suggests that the size and form of the sperm do not necessarily hold similar internal structures. Thus, the frequency of “normal” sperm significantly increased after ICSI selection, but the improvement was more marked in donor than in OA patients' samples. In conclusion, at least a twofold increase in the number of normal spermatozoa was achieved after ICSI selection. The heterogeneity in the stainability and chromatin condensation of epididymal samples from OA patients indicates that some of the selected spermatozoa have a hypocondensed or hypercondensed chromatin. Even in the best of donor cases, no more than 55% of the selected sperm scored normal with CKIA, indicating that the present routine ICSI selection criteria are not sufficient for selecting normal condensed nuclei.</p>\",\"PeriodicalId\":15029,\"journal\":{\"name\":\"Journal of andrology\",\"volume\":\"25 3\",\"pages\":\"406-411\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-01-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/j.1939-4640.2004.tb02807.x\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of andrology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/j.1939-4640.2004.tb02807.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of andrology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/j.1939-4640.2004.tb02807.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 26

摘要

摘要:本研究的目的是评估来自阻塞性无精子症(OA)患者的卵泡内单精子注射(ICSI)选择的附睾精子的核正常情况。我们评估了常规ICSI中使用的选择标准(400倍放大镜下的形态和活力)是否足以从附睾样本中选择“正常”精子。采用dna特异性染色(Feulgen)和计算机核成像分析(CKIA)系统对15例OA患者的手术取精和9例正常精子供者的射精样本进行评估。原始(未选择)样本和icsi选择的精子在供体和患者样本中进行比较。在原始分数中,附睾精子中几乎所有测量参数的变化都比射精精子大。精子选择后,附睾精子和射精精子的形态测量具有可比性。然而,对于与DNA染色和染色质结构(核凝聚)相关的参数,观察到患者和供者之间存在显著差异。这一结果表明,精子的大小和形态不一定具有相似的内部结构。因此,ICSI选择后“正常”精子的频率显著增加,但供体的改善比OA患者的样本更明显。总之,ICSI选择后,正常精子的数量至少增加了两倍。OA患者附睾样品染色和染色质凝聚的异质性表明,一些选择的精子染色质低凝聚或高凝聚。即使在最好的供体病例中,所选精子的CKIA评分正常的也不超过55%,这表明目前常规ICSI选择标准不足以选择正常凝聚核。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluation of ICSI-Selected Epididymal Sperm Samples of Obstructive Azoospermic Males by the CKIA System

Evaluation of ICSI-Selected Epididymal Sperm Samples of Obstructive Azoospermic Males by the CKIA System

ABSTRACT: The objective of this study was to evaluate nuclear normality in intracytoplasmic sperm injection (ICSI)-selected epididymal sperm from obstructive azoospermic (OA) patients. We evaluated whether the selection criteria used in routine ICSI (morphology and motility at a magnification of 400×) is adequate for selecting “normal” sperm from epididymal samples. Surgically retrieved spermatozoa from the caput epididymis of 15 OA patients and ejaculated sperm samples from 9 normospermic donors were evaluated with a DNA-specific stain (Feulgen) and in combination with the computerized karyometric image analysis (CKIA) system. Original (unselected) samples and ICSI-selected sperm were compared in donor and patient samples. In the original fraction, a larger variation in almost all measured parameters was found in epididymal sperm than in ejaculated sperm. After sperm selection, the morphometry was comparable between epididymal and ejaculated sperm. However, for those parameters related to the DNA stainability and chromatin texture (nuclear condensation), significant differences between patients and donors were observed. This result suggests that the size and form of the sperm do not necessarily hold similar internal structures. Thus, the frequency of “normal” sperm significantly increased after ICSI selection, but the improvement was more marked in donor than in OA patients' samples. In conclusion, at least a twofold increase in the number of normal spermatozoa was achieved after ICSI selection. The heterogeneity in the stainability and chromatin condensation of epididymal samples from OA patients indicates that some of the selected spermatozoa have a hypocondensed or hypercondensed chromatin. Even in the best of donor cases, no more than 55% of the selected sperm scored normal with CKIA, indicating that the present routine ICSI selection criteria are not sufficient for selecting normal condensed nuclei.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of andrology
Journal of andrology 医学-男科学
自引率
0.00%
发文量
0
审稿时长
5.6 months
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信