{"title":"川芎嗪对单层培养兔关节软骨细胞Bcl-2蛋白表达及细胞凋亡的影响。","authors":"Sheng Xiao, Kang-hua Li, Hong-Bin Lu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture.</p><p><strong>Methods: </strong>We separated chondrocytes from the rabbit articular cartilage and cultured them in vitro. We divided them into 2 groups. One group was added the injective ligustrazine hydrochloride(LH), and the other was added the same quantity of culture medium as the comparison. The expression of Bcl-2 in every group was examined with immunohistocytochemistry technique, and the apoptosis was observed with TUNEL.</p><p><strong>Results: </strong>The two groups both expressed Bcl-2 protein, but the expression in the LH group was significantly higher than that in the comparison group (P < 0.01). The two groups both had apoptosis, the rate of which in the LH group was significantly lower than that in the comparison group (P < 0.01).</p><p><strong>Conclusion: </strong>Ligustrazine may repair the cartilaginous lesion.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"224-6"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture].\",\"authors\":\"Sheng Xiao, Kang-hua Li, Hong-Bin Lu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To determine the effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture.</p><p><strong>Methods: </strong>We separated chondrocytes from the rabbit articular cartilage and cultured them in vitro. We divided them into 2 groups. One group was added the injective ligustrazine hydrochloride(LH), and the other was added the same quantity of culture medium as the comparison. The expression of Bcl-2 in every group was examined with immunohistocytochemistry technique, and the apoptosis was observed with TUNEL.</p><p><strong>Results: </strong>The two groups both expressed Bcl-2 protein, but the expression in the LH group was significantly higher than that in the comparison group (P < 0.01). The two groups both had apoptosis, the rate of which in the LH group was significantly lower than that in the comparison group (P < 0.01).</p><p><strong>Conclusion: </strong>Ligustrazine may repair the cartilaginous lesion.</p>\",\"PeriodicalId\":13115,\"journal\":{\"name\":\"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University\",\"volume\":\"28 3\",\"pages\":\"224-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture].
Objective: To determine the effect of ligustrazine on the expression of Bcl-2 protein and apoptosis in rabbit articular chondrocytes in monolayer culture.
Methods: We separated chondrocytes from the rabbit articular cartilage and cultured them in vitro. We divided them into 2 groups. One group was added the injective ligustrazine hydrochloride(LH), and the other was added the same quantity of culture medium as the comparison. The expression of Bcl-2 in every group was examined with immunohistocytochemistry technique, and the apoptosis was observed with TUNEL.
Results: The two groups both expressed Bcl-2 protein, but the expression in the LH group was significantly higher than that in the comparison group (P < 0.01). The two groups both had apoptosis, the rate of which in the LH group was significantly lower than that in the comparison group (P < 0.01).
Conclusion: Ligustrazine may repair the cartilaginous lesion.
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