{"title":"[TLR4 mRNA表达与lps诱导小鼠肝损伤的关系]。","authors":"Xiao-wei Liu, Yu You, Fang-gen Lu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).</p><p><strong>Methods: </strong>Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.</p><p><strong>Results: </strong>A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.</p><p><strong>Conclusion: </strong>The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 3","pages":"217-20"},"PeriodicalIF":0.0000,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[TLR4 mRNA expression and liver injury in LPS-induced mouse].\",\"authors\":\"Xiao-wei Liu, Yu You, Fang-gen Lu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).</p><p><strong>Methods: </strong>Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.</p><p><strong>Results: </strong>A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.</p><p><strong>Conclusion: </strong>The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.</p>\",\"PeriodicalId\":13115,\"journal\":{\"name\":\"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University\",\"volume\":\"28 3\",\"pages\":\"217-20\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[TLR4 mRNA expression and liver injury in LPS-induced mouse].
Objective: To determine the relationship between Toll-like receptor 4 (TLR4) mRNA expression and liver injury induced by lipopolysaccharide (LPS).
Methods: Balb/c mice were sacrificed at the indicated time points after the intra-peritoneal LPS. The livers were stained with hematoxylin and erosin for histopathologic analysis. Simultaneously, the expression of TLR4mRNA was detected by RT-PCR.
Results: A large number of inflammatory cells infiltrated into the portal areas and focal hepatocellular necrosis occurred 24 h after the intra-peritoneal LPS. The expression of TLR4mRNA in the liver was significantly depressed at 6 h and 12 h after the intra-peritoneal LPS, but recruited at 24 h.
Conclusion: The signal transduction molecule TLR4 plays an important role in LPS-induced liver injury.
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