{"title":"[柑桔提取物对豚鼠心室肌细胞l型钙电流的影响]。","authors":"Fang Fang, Min Dong, Hong Zhu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs.</p><p><strong>Methods: </strong>Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE.</p><p><strong>Results: </strong>1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves.</p><p><strong>Conclusions: </strong>CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel.</p>","PeriodicalId":13115,"journal":{"name":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","volume":"28 4","pages":"353-6"},"PeriodicalIF":0.0000,"publicationDate":"2003-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs].\",\"authors\":\"Fang Fang, Min Dong, Hong Zhu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs.</p><p><strong>Methods: </strong>Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE.</p><p><strong>Results: </strong>1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves.</p><p><strong>Conclusions: </strong>CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel.</p>\",\"PeriodicalId\":13115,\"journal\":{\"name\":\"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University\",\"volume\":\"28 4\",\"pages\":\"353-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs].
Objective: To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs.
Methods: Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE.
Results: 1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves.
Conclusions: CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel.
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