胡文毒素- iii神经毒性肽的纯化和鉴定及胡文毒素- iii的天然失活突变体。

Ren-Huai Huang, Zhong-Hua Liu, Song-Ping Liang
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引用次数: 0

摘要

一种新的神经毒性肽,命名为huwentoxin-III,并从一种天然突变体中分离出来。质谱法测定两肽的平均分子量分别为3853.35和3667.40。虎文毒素iii型含有33个氨基酸残基,其中含有6个半胱氨酸残基。它的自然突变体只是截断了huwentoxin-III的c端色氨酸残基。这两种肽的序列与先前从同一蜘蛛毒液中分离到的凝集素样肽SHL-I的序列相似度为70.5%,但它们不能凝集人红细胞。毒蛾毒素iii能使蟑螂可逆瘫痪数小时,其ED(50)为(192.95 +/- 120.84)μ g/g (P=0.95)(平均+/- SD),并能增强离体大鼠输精管神经引起的肌肉收缩,而毒蛾毒素iii突变体无此作用,提示Trp33是毒蛾毒素iii生物学功能相关的重要残基。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Purification and characterization of a neurotoxic peptide huwentoxin-III and a natural inactive mutant from the venom of the spider Selenocosmia huwena Wang (Ornithoctonus huwena Wang).

A novel neurotoxic peptide, named huwentoxin-III, and a natural mutant have been isolated from the venom of the spider Selenocosmia huwena Wang (Ornithoctonus huwena Wang). The average molecular weights of the two peptides were determined as 3853.35 and 3667.40 by mass spectrometry, respectively. Huwentoxin-III has 33 amino acid residues containing 6 cysteine residues. Its natural mutant is only truncated a tryptophan residue from C-terminals of huwentoxin-III. The sequences of the two peptides show 70.5% sequence similarity with that of lectin-like peptide SHL-I previously isolated from the venom of the same spider, while they cannot agglutinate human erythrocytes. Huwentoxin-III can reversibly paralyze cockroaches for several hours with an ED(50) of (192.95 +/- 120.84) microg/g (P=0.95) (mean +/- SD) and can enhance the muscular contractions elicited by stimulating the nerve of the isolated rat vas deferens, however, the mutant of huwentoxin-III has no such effect, which suggests that Trp33 was an important residue related to the biological function of huwentoxin-III.

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