Andreas Leonhardt , Alexander Glaser , Markus Wegmann , Reinhard Hackenberg , Rolf M Nüsing
{"title":"前列腺素受体在人妊娠下段肌层中的表达。","authors":"Andreas Leonhardt , Alexander Glaser , Markus Wegmann , Reinhard Hackenberg , Rolf M Nüsing","doi":"10.1016/S0952-3278(03)00113-3","DOIUrl":null,"url":null,"abstract":"<div><div><span>Prostanoids, especially prostaglandin (PG) E</span><sub>2</sub><span><span><span>, are important mediators of uterine relaxation<span> and contractions during gestation<span> and parturition. Inhibitors of </span></span></span>PG formation as well as </span>PG analogues<span><span> are used to modulate uterine tonus. So far, only limited data are available regarding the expression of prostanoid receptors in human pregnant </span>myometrium. In the present study, the expression of the receptors for PGE</span></span><sub>2</sub> (EP1, EP2, EP3, EP4), PGF<sub>2<em>α</em></sub><span> (FP), prostacyclin (IP), and thromboxane A</span><sub>2</sub><span><span><span> (TP) in human pregnant myometrium was studied by RT-PCR, </span>in situ hybridization and </span>immunohistochemistry<span>. Myometrial tissue was obtained from five women at term and not in labour and from two women who delivered preterm. Tissue specimens were excised from the upper edge of the transverse lower uterine segment incision. In all tissues analysed, EP1, EP2, EP3, EP4, FP, TP and IP receptor mRNA and protein was detected. mRNA expression for PGD</span></span><sub>2</sub><span> (DP) receptor was not detected in the majority of tissue specimens. EP1, EP2, EP4, IP, TP and FP receptor protein<span> was detected on myometrial smooth muscle cells<span><span>, whereas EP3 receptor protein was only expressed by stromal and </span>endothelial cells. In situ hybridization experiments yielded similar results. The expression of the EP2 receptor mRNA was inversely related to gestational age. We suggest that the contractile effect of PGE</span></span></span><sub>2</sub><span><span><span> at term is probably mediated directly by the EP1 receptor expressed in myometrial </span>smooth muscle cells and indirectly by the EP3 receptor expressed in </span>stromal cells<span> and a decrease in EP2 receptor expression.</span></span></div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"69 5","pages":"Pages 307-313"},"PeriodicalIF":3.0000,"publicationDate":"2003-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression of prostanoid receptors in human lower segment pregnant myometrium\",\"authors\":\"Andreas Leonhardt , Alexander Glaser , Markus Wegmann , Reinhard Hackenberg , Rolf M Nüsing\",\"doi\":\"10.1016/S0952-3278(03)00113-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><span>Prostanoids, especially prostaglandin (PG) E</span><sub>2</sub><span><span><span>, are important mediators of uterine relaxation<span> and contractions during gestation<span> and parturition. Inhibitors of </span></span></span>PG formation as well as </span>PG analogues<span><span> are used to modulate uterine tonus. So far, only limited data are available regarding the expression of prostanoid receptors in human pregnant </span>myometrium. In the present study, the expression of the receptors for PGE</span></span><sub>2</sub> (EP1, EP2, EP3, EP4), PGF<sub>2<em>α</em></sub><span> (FP), prostacyclin (IP), and thromboxane A</span><sub>2</sub><span><span><span> (TP) in human pregnant myometrium was studied by RT-PCR, </span>in situ hybridization and </span>immunohistochemistry<span>. Myometrial tissue was obtained from five women at term and not in labour and from two women who delivered preterm. Tissue specimens were excised from the upper edge of the transverse lower uterine segment incision. In all tissues analysed, EP1, EP2, EP3, EP4, FP, TP and IP receptor mRNA and protein was detected. mRNA expression for PGD</span></span><sub>2</sub><span> (DP) receptor was not detected in the majority of tissue specimens. EP1, EP2, EP4, IP, TP and FP receptor protein<span> was detected on myometrial smooth muscle cells<span><span>, whereas EP3 receptor protein was only expressed by stromal and </span>endothelial cells. In situ hybridization experiments yielded similar results. The expression of the EP2 receptor mRNA was inversely related to gestational age. We suggest that the contractile effect of PGE</span></span></span><sub>2</sub><span><span><span> at term is probably mediated directly by the EP1 receptor expressed in myometrial </span>smooth muscle cells and indirectly by the EP3 receptor expressed in </span>stromal cells<span> and a decrease in EP2 receptor expression.</span></span></div></div>\",\"PeriodicalId\":94179,\"journal\":{\"name\":\"Prostaglandins, leukotrienes, and essential fatty acids\",\"volume\":\"69 5\",\"pages\":\"Pages 307-313\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2003-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins, leukotrienes, and essential fatty acids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0952327803001133\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and essential fatty acids","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0952327803001133","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Expression of prostanoid receptors in human lower segment pregnant myometrium
Prostanoids, especially prostaglandin (PG) E2, are important mediators of uterine relaxation and contractions during gestation and parturition. Inhibitors of PG formation as well as PG analogues are used to modulate uterine tonus. So far, only limited data are available regarding the expression of prostanoid receptors in human pregnant myometrium. In the present study, the expression of the receptors for PGE2 (EP1, EP2, EP3, EP4), PGF2α (FP), prostacyclin (IP), and thromboxane A2 (TP) in human pregnant myometrium was studied by RT-PCR, in situ hybridization and immunohistochemistry. Myometrial tissue was obtained from five women at term and not in labour and from two women who delivered preterm. Tissue specimens were excised from the upper edge of the transverse lower uterine segment incision. In all tissues analysed, EP1, EP2, EP3, EP4, FP, TP and IP receptor mRNA and protein was detected. mRNA expression for PGD2 (DP) receptor was not detected in the majority of tissue specimens. EP1, EP2, EP4, IP, TP and FP receptor protein was detected on myometrial smooth muscle cells, whereas EP3 receptor protein was only expressed by stromal and endothelial cells. In situ hybridization experiments yielded similar results. The expression of the EP2 receptor mRNA was inversely related to gestational age. We suggest that the contractile effect of PGE2 at term is probably mediated directly by the EP1 receptor expressed in myometrial smooth muscle cells and indirectly by the EP3 receptor expressed in stromal cells and a decrease in EP2 receptor expression.