{"title":"核糖核酸定量沉淀用硫酸鱼精蛋白的纯化","authors":"Georg R. Philipps, Jane West","doi":"10.1016/0926-6550(64)90071-4","DOIUrl":null,"url":null,"abstract":"<div><p>A method is described for the purification of protamine sulfate by chromatography on DEAE-cellulose. The precipitation of soluble RNA and ribosomal RNA by purified protamine is quantitative, while oligonucleotides of low molecular weight are not precipitated. The procedure allows the quantitative removal of soluble RNA from solutions and the preparation of soluble RNA samples of high purity.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 3","pages":"Pages 416-420"},"PeriodicalIF":0.0000,"publicationDate":"1964-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90071-4","citationCount":"4","resultStr":"{\"title\":\"Purification of protamine sulfate for the quantitative precipitation of ribonucleic acids\",\"authors\":\"Georg R. Philipps, Jane West\",\"doi\":\"10.1016/0926-6550(64)90071-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A method is described for the purification of protamine sulfate by chromatography on DEAE-cellulose. The precipitation of soluble RNA and ribosomal RNA by purified protamine is quantitative, while oligonucleotides of low molecular weight are not precipitated. The procedure allows the quantitative removal of soluble RNA from solutions and the preparation of soluble RNA samples of high purity.</p></div>\",\"PeriodicalId\":100173,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"volume\":\"91 3\",\"pages\":\"Pages 416-420\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6550(64)90071-4\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926655064900714\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900714","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Purification of protamine sulfate for the quantitative precipitation of ribonucleic acids
A method is described for the purification of protamine sulfate by chromatography on DEAE-cellulose. The precipitation of soluble RNA and ribosomal RNA by purified protamine is quantitative, while oligonucleotides of low molecular weight are not precipitated. The procedure allows the quantitative removal of soluble RNA from solutions and the preparation of soluble RNA samples of high purity.
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