酪蛋白的研究7。从κ-酪蛋白酶解物中分离的糖肽类物质的碳水化合物构成

F.Y-Y. Huang, G.O. Henneberry, B.E. Baker
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引用次数: 8

摘要

用结晶木瓜蛋白酶(EC 3.4.4.10)或蛋白酶p对κ-酪蛋白进行水解,水解产物用离子交换和Sephadex G-25进行分离。从木瓜蛋白酶消化产物中分离出富含碳水化合物的巨肽,其己糖、己糖胺和唾液酸的含量是κ-酪蛋白的4-5倍。在κ-酪蛋白中,这三种糖的浓度与它们在巨肽部分中的浓度比例大致相同。这些结果表明,κ-酪蛋白的碳水化合物部分可能是一个由己糖、己糖胺和唾液酸按1:1:1的比例组成的假基。从Pronase-P水解产物中分离出来的大肽部分所含的碳水化合物大约是从木瓜蛋白酶消化产物中分离出来的大肽的两倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies of casein VII. The carbohydrate constitution of glycopeptidic material isolated from enzymic hydrolysates of κ-casein

κ-Casein was hydrolyzed with either crystalline papain (EC 3.4.4.10) or Pronase P. The products of hydrolysis were fractionated by means of ion exchange and Sephadex G-25. A carbohydrate-rich macropeptide isolated from the papain digest contained 4–5 times the quantities of hexose, hexosamine and sialic acid that were present in κ-casein. The concentrations of the three saccharides bore approximately the same ratio to each other in κ-casein as they did in the macropeptide fraction. These results suggested that the carbohydrate part of κ-casein may be a prosthetic group containing hexose, hexosamine and sialic acid in the ratio of 1:1:1. The macropeptide fraction isolated from the Pronase-P hydrolyate contained approximately double the quantity of carbohydrate that was present in the macropeptide isolated from the papain digest.

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