浓盐酸水解几丁质，制备低分子量溶菌酶底物

Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides Pub Date : 1964-11-01 DOI:10.1016/0926-6526(64)90001-1

J.A. Rupley

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引用次数: 414

摘要

研究了几丁质在强酸溶液中在一定浓度和温度下的水解过程。数据表明，水解速率由甲壳素的结构决定。开发了一种从几丁质中分离寡糖的简单方法，用于制备低分子量溶菌酶底物(n -乙酰muramide glycanohydrolase, EC 3.2.1.17)。研究溶菌酶催化水解这些低聚糖的实验表明，链长度对水解速率有很大影响，而离子强度对水解速率没有影响。

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The hydrolysis of chitin by concentrated hydrochloric acid, and the preparation of low-molecular-weight substrate for lysozyme

The hydrolysis of chitin in solution in strong HCl was studied over a range of acid concentration and temperature. The data suggest that the rate of hydrolysis governed by the structure of the chitin. A simple procedure for the isolation of the oligosaccharides obtained from chitin was developed, for use in the preparation of low-molecular-weight substrates for lysozyme (N-acetylmuramide glycanohydrolase, EC 3.2.1.17). Experiments studying the lysozyme-catalyzed hydrolysis of these oligosaccharides showed a large effect of chain length upon the rate of hydrolysis, and no effect of ionic strength.

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Mucoproteins and Mucopolysaccharides

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