{"title":"分离肝细胞对乳糜微粒脂肪酸的摄取","authors":"C. Green, Joan A. Webb","doi":"10.1016/0926-6542(64)90004-6","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Rats were fed [<sup>14</sup>C]tripalmitin and guinea-pigs injected with <sup>14</sup>C-labelled chylomicrons and the livers examined at various time intervals. Most of the <sup>14</sup>C was associated with the individual liver cells but up to 43% of the total in the tissue could be free of the cells.</p></span></li><li><span>2.</span><span><p>2. Isolated rat-liver cells can bind chylomicrons to the extent of 40 μg lipid per mg tissue N.</p></span></li><li><span>3.</span><span><p>3. The bound chylomicrons are hydrolysed to give free fatty acids.</p></span></li><li><span>4.</span><span><p>4. The fatty acids produced are firmly bound but can be removed by fatty acid-free albumin, suggesting that hydrolysis occurs at the cell surface.</p></span></li><li><span>5.</span><span><p>5. The hydrolysis of chylomicron triglyceride is not inhibited by protamine sulphate or by heating the cells at 60°; nor is it stimulated by taurocholate.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 4","pages":"Pages 404-411"},"PeriodicalIF":0.0000,"publicationDate":"1964-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90004-6","citationCount":"23","resultStr":"{\"title\":\"The uptake of chylomicron fatty acids by isolated liver cells\",\"authors\":\"C. Green, Joan A. Webb\",\"doi\":\"10.1016/0926-6542(64)90004-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Rats were fed [<sup>14</sup>C]tripalmitin and guinea-pigs injected with <sup>14</sup>C-labelled chylomicrons and the livers examined at various time intervals. Most of the <sup>14</sup>C was associated with the individual liver cells but up to 43% of the total in the tissue could be free of the cells.</p></span></li><li><span>2.</span><span><p>2. Isolated rat-liver cells can bind chylomicrons to the extent of 40 μg lipid per mg tissue N.</p></span></li><li><span>3.</span><span><p>3. The bound chylomicrons are hydrolysed to give free fatty acids.</p></span></li><li><span>4.</span><span><p>4. The fatty acids produced are firmly bound but can be removed by fatty acid-free albumin, suggesting that hydrolysis occurs at the cell surface.</p></span></li><li><span>5.</span><span><p>5. The hydrolysis of chylomicron triglyceride is not inhibited by protamine sulphate or by heating the cells at 60°; nor is it stimulated by taurocholate.</p></span></li></ul></div>\",\"PeriodicalId\":100171,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"volume\":\"84 4\",\"pages\":\"Pages 404-411\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-08-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6542(64)90004-6\",\"citationCount\":\"23\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926654264900046\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926654264900046","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The uptake of chylomicron fatty acids by isolated liver cells
1.
1. Rats were fed [14C]tripalmitin and guinea-pigs injected with 14C-labelled chylomicrons and the livers examined at various time intervals. Most of the 14C was associated with the individual liver cells but up to 43% of the total in the tissue could be free of the cells.
2.
2. Isolated rat-liver cells can bind chylomicrons to the extent of 40 μg lipid per mg tissue N.
3.
3. The bound chylomicrons are hydrolysed to give free fatty acids.
4.
4. The fatty acids produced are firmly bound but can be removed by fatty acid-free albumin, suggesting that hydrolysis occurs at the cell surface.
5.
5. The hydrolysis of chylomicron triglyceride is not inhibited by protamine sulphate or by heating the cells at 60°; nor is it stimulated by taurocholate.
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