{"title":"尼古丁对电激电位的作用及其电离型和非电离型电激电位的差异","authors":"Eva Bartels, Thomas R. Podleski","doi":"10.1016/0926-6577(64)90216-5","DOIUrl":null,"url":null,"abstract":"<div><p>The effect of nicotine and the pH dependence of its potency were tested on the monocellular electroplax preparation of Electrophorus.</p><ul><li><span><p>1. Nicotine (5·10<sup>−5</sup>M) blocks the action potential reversibly within 5 min. The stepwise depolarization of the membrane by increasing concentrations is shown.</p></span></li><li><span><p>2. Curare inhibits the effect of nicotine. Both compounds act at the synapse only. The mode of action is competitive. In contrast, the toxin obtained from clam affects the preparation without interference with the effect of nicotine.</p></span></li><li><span><p>3. Increasing the pH from 6.2 to 9.0 decreases the potency of nicotine by a factor of 2–3 and, in addition, increases the time required for reaching the equilibrium potential by a factor of 5–10.</p></span></li><li><span><p>4. The degree of ionization of nicotine has been evaluated in relation to potency of action.</p></span></li></ul></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1964-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90216-5","citationCount":"13","resultStr":"{\"title\":\"Action of nicotine on the electroplax and difference of potency between ionized and unionized forms\",\"authors\":\"Eva Bartels, Thomas R. Podleski\",\"doi\":\"10.1016/0926-6577(64)90216-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The effect of nicotine and the pH dependence of its potency were tested on the monocellular electroplax preparation of Electrophorus.</p><ul><li><span><p>1. Nicotine (5·10<sup>−5</sup>M) blocks the action potential reversibly within 5 min. The stepwise depolarization of the membrane by increasing concentrations is shown.</p></span></li><li><span><p>2. Curare inhibits the effect of nicotine. Both compounds act at the synapse only. The mode of action is competitive. In contrast, the toxin obtained from clam affects the preparation without interference with the effect of nicotine.</p></span></li><li><span><p>3. Increasing the pH from 6.2 to 9.0 decreases the potency of nicotine by a factor of 2–3 and, in addition, increases the time required for reaching the equilibrium potential by a factor of 5–10.</p></span></li><li><span><p>4. The degree of ionization of nicotine has been evaluated in relation to potency of action.</p></span></li></ul></div>\",\"PeriodicalId\":100169,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6577(64)90216-5\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926657764902165\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764902165","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Action of nicotine on the electroplax and difference of potency between ionized and unionized forms
The effect of nicotine and the pH dependence of its potency were tested on the monocellular electroplax preparation of Electrophorus.
1. Nicotine (5·10−5M) blocks the action potential reversibly within 5 min. The stepwise depolarization of the membrane by increasing concentrations is shown.
2. Curare inhibits the effect of nicotine. Both compounds act at the synapse only. The mode of action is competitive. In contrast, the toxin obtained from clam affects the preparation without interference with the effect of nicotine.
3. Increasing the pH from 6.2 to 9.0 decreases the potency of nicotine by a factor of 2–3 and, in addition, increases the time required for reaching the equilibrium potential by a factor of 5–10.
4. The degree of ionization of nicotine has been evaluated in relation to potency of action.
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