体内叶绿素对丙酮处理的吸收响应

J.B. Thomas, U.P. Van Der Wal
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引用次数: 12

摘要

用加入不同浓度丙酮的缓冲液处理蜘蛛叶绿体。当用缓冲液代替含溶剂介质时,高达40%的丙酮浓度似乎会产生主吸收最大值向短波侧的可逆移动。在浓度较高的情况下,这种转变至少部分是不可逆的。这种变化的幅度与丙酮浓度成线性关系。浓度低于50%的丙酮最有可能溶解叶绿素-蛋白质复合物。这些配合物在含丙酮介质中的主要吸收最大值的位置被移到短波侧,然而,同样在这种情况下,移动的幅度取决于所应用的溶剂浓度。至少有一些单独的叶绿素a类型被不同浓度的丙酮以相互不同的速率提取。后一个结果强调了这些叶绿素a类型的个性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Absorption responses of chlorophyll in vivo to treatment with acetone

Aspidistra elatior chloroplasts are treated with buffer to which acetone of various concentrations is added.

Upon replacement of the solvent-containing medium by buffer, it appears that acetone concentrations up to about 40% produce a reversible shift of the main absorption maximum to the short-wave side. With higher concentrations, such a shift is, least partially, irreversible. The magnitude of this shift proceeds linearly with acetone concentration.

Acetone in concentrations lower than about 50% most probably solubilizes chlorophyll-protein complexes. The position of the main absorption maximum of these complexes in the acetone-containing medium is shifted to the short-wave side, whereas, also in this case, the magnitude of the shift is dependent on the applied solvent concentration.

At least some of the individual chlorophyll a types are extracted by acetone of various concentrations at mutually different rates. The latter result stresses the individuality of these chlorophyll a types.

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