大环内酯类抗生素抑制人白细胞前列腺素E2合成及前列腺素合成酶mRNA表达。

IF 3
Michiko Miyazaki, Masafumi Zaitsu, Kinji Honjo, Eiichi Ishii, Yuhei Hamasaki
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引用次数: 0

摘要

我们研究了被认为具有抗炎活性的大环内酯类抗生素对脂多糖(LPS)刺激的前列腺素(PG) E2合成和人白细胞胞质磷脂酶A2 (cPLA2)、环氧化酶(COX)-1和COX-2 mrna表达的作用。lps刺激的外周多形核白细胞(PMNLs)和单核白细胞(MNLs)中PGE2的产生显著增加。LPS在PMNLs和MNLs中增加了COX-2和cPLA2 mrna的数量,但没有增加COX-1 mrna的数量。克拉霉素、阿奇霉素和地塞米松抑制了lps增强的PGE2合成以及cPLA2和COX-2 mrna的表达。克拉霉素和阿奇霉素可降低PMNLs中COX-1 mRNA的表达。大环内酯类抗生素通过抑制cPLA2、COX-1和COX-2 mRNA表达抑制人白细胞PGE2合成。这些数据提示了大环内酯类抗炎活性的一种机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Macrolide antibiotics inhibit prostaglandin E2 synthesis and mRNA expression of prostaglandin synthetic enzymes in human leukocytes
We investigated the action of macrolide antibiotics, which are considered to have anti-inflammatory activity, on lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E2 synthesis and the expression of mRNAs for cytosolic phospholipase A2 (cPLA2), cyclooxygenase (COX)-1, and COX-2 in human leukocytes. The production of LPS-stimulated PGE2 was significantly increased in peripheral polymorphonuclear leukocytes (PMNLs) and in mononuclear leukocytes (MNLs). Amounts of mRNAs for COX-2 and cPLA2, but not for COX-1, were enhanced by LPS in PMNLs and MNLs. The LPS-enhanced PGE2 synthesis and the expression of cPLA2 and COX-2 mRNAs were inhibited by clarithromycin, azithromycin and dexamethasone in PMNLs and MNLs. The mRNA expression of COX-1 in PMNLs was decreased by clarithromycin and azithromycin. Macrolide antibiotics inhibited PGE2 synthesis in human leukocytes by suppressing cPLA2, COX-1, and COX-2 mRNA expression. These data indicate one mechanism of macrolide anti-inflammatory activity.
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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
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审稿时长
64 days
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