{"title":"大环内酯类抗生素抑制人白细胞前列腺素E2合成及前列腺素合成酶mRNA表达。","authors":"Michiko Miyazaki, Masafumi Zaitsu, Kinji Honjo, Eiichi Ishii, Yuhei Hamasaki","doi":"10.1016/S0952-3278(03)00089-9","DOIUrl":null,"url":null,"abstract":"<div><div><span><span><span>We investigated the action of macrolide </span>antibiotics, which are considered to have anti-inflammatory activity, on </span>lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E</span><sub>2</sub> synthesis and the expression of mRNAs for cytosolic phospholipase A<sub>2</sub> (cPLA<sub>2</sub>), cyclooxygenase (COX)-1, and COX-2 in human leukocytes. The production of LPS-stimulated PGE<sub>2</sub> was significantly increased in peripheral polymorphonuclear leukocytes (PMNLs) and in mononuclear leukocytes (MNLs). Amounts of mRNAs for COX-2 and cPLA<sub>2</sub>, but not for COX-1, were enhanced by LPS in PMNLs and MNLs. The LPS-enhanced PGE<sub>2</sub> synthesis and the expression of cPLA<sub>2</sub><span> and COX-2 mRNAs were inhibited by clarithromycin<span><span>, azithromycin and </span>dexamethasone in PMNLs and MNLs. The mRNA expression of COX-1 in PMNLs was decreased by clarithromycin and azithromycin. Macrolide antibiotics inhibited PGE</span></span><sub>2</sub> synthesis in human leukocytes by suppressing cPLA<sub>2</sub>, COX-1, and COX-2 mRNA expression. These data indicate one mechanism of macrolide anti-inflammatory activity.</div></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"69 4","pages":"Pages 229-235"},"PeriodicalIF":3.0000,"publicationDate":"2003-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Macrolide antibiotics inhibit prostaglandin E2 synthesis and mRNA expression of prostaglandin synthetic enzymes in human leukocytes\",\"authors\":\"Michiko Miyazaki, Masafumi Zaitsu, Kinji Honjo, Eiichi Ishii, Yuhei Hamasaki\",\"doi\":\"10.1016/S0952-3278(03)00089-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><span><span><span>We investigated the action of macrolide </span>antibiotics, which are considered to have anti-inflammatory activity, on </span>lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E</span><sub>2</sub> synthesis and the expression of mRNAs for cytosolic phospholipase A<sub>2</sub> (cPLA<sub>2</sub>), cyclooxygenase (COX)-1, and COX-2 in human leukocytes. The production of LPS-stimulated PGE<sub>2</sub> was significantly increased in peripheral polymorphonuclear leukocytes (PMNLs) and in mononuclear leukocytes (MNLs). Amounts of mRNAs for COX-2 and cPLA<sub>2</sub>, but not for COX-1, were enhanced by LPS in PMNLs and MNLs. The LPS-enhanced PGE<sub>2</sub> synthesis and the expression of cPLA<sub>2</sub><span> and COX-2 mRNAs were inhibited by clarithromycin<span><span>, azithromycin and </span>dexamethasone in PMNLs and MNLs. The mRNA expression of COX-1 in PMNLs was decreased by clarithromycin and azithromycin. Macrolide antibiotics inhibited PGE</span></span><sub>2</sub> synthesis in human leukocytes by suppressing cPLA<sub>2</sub>, COX-1, and COX-2 mRNA expression. These data indicate one mechanism of macrolide anti-inflammatory activity.</div></div>\",\"PeriodicalId\":94179,\"journal\":{\"name\":\"Prostaglandins, leukotrienes, and essential fatty acids\",\"volume\":\"69 4\",\"pages\":\"Pages 229-235\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2003-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins, leukotrienes, and essential fatty acids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0952327803000899\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and essential fatty acids","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0952327803000899","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Macrolide antibiotics inhibit prostaglandin E2 synthesis and mRNA expression of prostaglandin synthetic enzymes in human leukocytes
We investigated the action of macrolide antibiotics, which are considered to have anti-inflammatory activity, on lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E2 synthesis and the expression of mRNAs for cytosolic phospholipase A2 (cPLA2), cyclooxygenase (COX)-1, and COX-2 in human leukocytes. The production of LPS-stimulated PGE2 was significantly increased in peripheral polymorphonuclear leukocytes (PMNLs) and in mononuclear leukocytes (MNLs). Amounts of mRNAs for COX-2 and cPLA2, but not for COX-1, were enhanced by LPS in PMNLs and MNLs. The LPS-enhanced PGE2 synthesis and the expression of cPLA2 and COX-2 mRNAs were inhibited by clarithromycin, azithromycin and dexamethasone in PMNLs and MNLs. The mRNA expression of COX-1 in PMNLs was decreased by clarithromycin and azithromycin. Macrolide antibiotics inhibited PGE2 synthesis in human leukocytes by suppressing cPLA2, COX-1, and COX-2 mRNA expression. These data indicate one mechanism of macrolide anti-inflammatory activity.