酵母双杂交法检测C17orf25与adp核糖焦磷酸酶NUDT9的相互作用。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-08-01

Hui-Tang Zhang, Zhi-Qiang Yan, Xiao-Bo Hu, Sheng-Li Yang, Yi Gong

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摘要

采用RACE PCR从肝脏中分离到C17orf25基因。采用酵母双杂交法从MatchMaker人HeLa cDNA文库中筛选出nudt9基因。NUDT9是一种以adp核糖为底物具有焦磷酸酶活性的酶。C17orf25与GFP的融合表达和计算机分析表明C17orf25可能位于线粒体中。此外，C17orf25可能通过与NUDT9相互作用抑制细胞生长。

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Interaction of C17orf25 with ADP-ribose pyrophosphatase NUDT9 detected via yeast two-hybrid method.

The gene C17orf25 was isolated from the liver by RACE PCR. nudt9 gene was screened by yeast two-hybrid method in MatchMaker human HeLa cDNA library. NUDT9 is an enzyme that has pyrophosphatase activity with ADP-ribose as its substrate. Fusion expression of C17orf25 and GFP and computer analysis showed that C17orf25 was probably located in mitochondria. Furthermore, C17orf25 may suppress the cell growth by interaction with NUDT9.

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来源期刊

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

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