[聚胞蓝藻PCC6803中疏水NAD(P)H脱氢酶亚复合物的分离]。

Yong Deng, Ji-Yu Ye, Hua-Ling Mi, Yun-Gang Shen
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引用次数: 0

摘要

对NA(D)PH脱氢酶(NDH)络合物的分离已经做了很多研究。从聚胞蓝藻PCC6803中纯化了NDH的几个亲水性亚配合物。然而,目前还没有从蓝藻中分离出疏水性NDH亚复合物。采用阴离子交换层析和凝胶过滤的方法,从聚囊藻PCC6803全细胞提取物中分离出两个NDH亚配合物。这两个亚配合物都含有疏水亚基NdhA,表明它们是疏水NDH亚配合物。在两个亚复合物中,只有一个亚复合物含有NdhH。这些亚配合物具有NADPH-硝基蓝四唑(NBT)氧化还原酶活性,当几种醌类似物作为电子受体时,如铁氰化物、2,5-二溴-3-甲基-6-异丙基-对苯醌(DBMIB)、2,6-二氯酚吲哚酚(DCPIP)、杜洛醌、泛醌-0 (UQ-0)等,可以特异性氧化NADPH。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Separation of hydrophobic NAD(P)H dehydrogenase subcomplexes from cyanobacterium Synechocystis PCC6803].

Many efforts have been paid to the separation of an integrated NA(D)PH dehydrogenase (NDH) complex. Several hydrophilic subcomplexes of NDH have been purified from the cyanobacterium Synechocystis PCC6803. However, no hydrophobic NDH subcomplex has ever been separated from cyanobacteria yet. In this paper, two NDH subcomplexes were separated from n-dodecyl beta-D-maltoside(DM)-treated whole cell extracts of Synechocystis PCC6803 by anion exchange chromatography and gel filtration. Both subcomplexes contained the hydrophobic subunit NdhA, suggesting that they were hydrophobic NDH subcomplexes. Of the two subcomplexes, only one subcomplex contained NdhH. These subcomplexes showed NADPH-nitroblue tetrazolium (NBT) oxidoreductase activity and could specifically oxidize NADPH when several quinone analogues were used as electron acceptors, such as ferricyanide, 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), 2,6-dichlorophenol indophenol (DCPIP), duroquinone, ubiquinone-0 (UQ-0), etc.

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