Magdalena Modrzewska, Paweł Karpiński, Anna Grudniak, Krystyna I Wolska
{"title":"DNA ak和dnaJ基因零突变对大肠杆菌偶联DNA转移、蛋白水解和新生物素敏感性的影响。","authors":"Magdalena Modrzewska, Paweł Karpiński, Anna Grudniak, Krystyna I Wolska","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Escherichia coli null dnaJ and dnaKdnaJ mutants, when introduced to Hfr donor, impair its ability to DNA transfer during conjugation. The additive effect of both mutations was shown. Lack of DnaK and DnaJ chaperones also decrease the extent of proteolysis in mutant strains. This effect is seen only at 42 degrees C. The influence of double dnaKdnaJ deletion but not single dnaJ deletion on novobiocin susceptibility was also demonstrated.</p>","PeriodicalId":75388,"journal":{"name":"Acta microbiologica Polonica","volume":"51 3","pages":"217-24"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of null mutations in dnaK and dnaJ genes on conjugational DNA transfer, proteolysis and novobiocin susceptibility of Escherichia coli.\",\"authors\":\"Magdalena Modrzewska, Paweł Karpiński, Anna Grudniak, Krystyna I Wolska\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Escherichia coli null dnaJ and dnaKdnaJ mutants, when introduced to Hfr donor, impair its ability to DNA transfer during conjugation. The additive effect of both mutations was shown. Lack of DnaK and DnaJ chaperones also decrease the extent of proteolysis in mutant strains. This effect is seen only at 42 degrees C. The influence of double dnaKdnaJ deletion but not single dnaJ deletion on novobiocin susceptibility was also demonstrated.</p>\",\"PeriodicalId\":75388,\"journal\":{\"name\":\"Acta microbiologica Polonica\",\"volume\":\"51 3\",\"pages\":\"217-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta microbiologica Polonica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Polonica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of null mutations in dnaK and dnaJ genes on conjugational DNA transfer, proteolysis and novobiocin susceptibility of Escherichia coli.
Escherichia coli null dnaJ and dnaKdnaJ mutants, when introduced to Hfr donor, impair its ability to DNA transfer during conjugation. The additive effect of both mutations was shown. Lack of DnaK and DnaJ chaperones also decrease the extent of proteolysis in mutant strains. This effect is seen only at 42 degrees C. The influence of double dnaKdnaJ deletion but not single dnaJ deletion on novobiocin susceptibility was also demonstrated.
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