Kai Hu , Jiamin Cheng , Kangbin Wang , Yuanqing Zhao , Yanju Liu , Huaixia Yang , Zhenqiang Zhang
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引用次数: 26
摘要
细胞角蛋白片段抗原21-1 (CYFRA21-1)是检测非小细胞肺癌(NSCLC)的敏感标志物。首次合成了金纳米粒子(AuNPs)和二硫化钼(MoS2)改性Ti3C2Tx，得到了AuNPs@MoS2@Ti3C2Tx复合材料，该复合材料具有较大的比表面积和良好的电催化性能。通过在材料表面加载大量二抗(Ab2)和甲苯胺蓝(TB)作为信号探针，以Nafion-AuNPs混合物作为电极材料，建立了一种新的检测CYFRA21-1的电化学免疫分析法。当CYFRA21-1的电化学响应值在0.5 pg mL−1 ~ 50 ng mL−1的浓度范围内线性增加时，检出限可低至0.03 pg mL−1。此外，实验结果表明，该生物传感器具有快速检测患者血清等复杂样品中CYFRA21-1的潜力，在NSCLC的早期诊断和监测中具有广阔的应用前景。
Sensitive electrochemical immunosensor for CYFRA21-1 detection based on AuNPs@MoS2@Ti3C2Tx composites
Cytokeratin fragment antigen 21–1 (CYFRA21-1) is a sensitive marker for detecting non-small cell lung cancer (NSCLC). Ti3C2Tx modified by gold nanoparticles (AuNPs) and molybdenum disulfide (MoS2) were synthesized for the first time to obtain the AuNPs@MoS2@Ti3C2Tx composites, which have large specific surface area and good electrocatalytic properties. A novel electrochemical immunoassay for sensitive detection of CYFRA21-1 was developed by loading a large quantity of secondary antibodies (Ab2) and toluidine blue (TB) on the surface of the material as signal probe, and Nafion-AuNPs mixture as electrode material. When the electrochemical response value of CYFRA21-1 increased linearly within the concentration range of 0.5 pg mL−1–50 ng mL−1, the detection limit can reach as low as 0.03 pg mL−1. In addition, the experimental results showed that the biosensor had the potential to rapidly detect CYFRA21-1 in the complex samples such as patient serum, and had a broad application prospect in the early diagnosis and monitoring of NSCLC.
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Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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