分子印迹聚合物对双糖识别的研究。

S Striegler
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引用次数: 20

摘要

研究了碳水化合物印迹聚合物对几种双糖的选择性,即纤维素二糖、麦芽糖、乳糖和gentiobose。在碱性溶液中形成三元配体- cu (II)-碳水化合物复合物,并在聚合物中捕获。在无缓冲、中性pH和20℃的水溶液中,在饱和条件下进行重结合实验,采用HPLC分析、折射法和1H NMR谱法研究了聚合物基质对双糖的可及性。结果表明,纤维素二糖和麦芽糖的-糖苷键和-糖苷键具有选择性。进一步表明,二糖印迹聚合物对纤维素二糖和gentiobose的1,4- β -和1,6- β -糖苷键有轻微的区分,而对纤维素二糖和乳糖没有选择性识别。由于功能Cu(II)单体在生理pH下与目标双糖的表观结合常数较弱,因此在应用条件下,识别过程主要受所形成的印迹腔的形状支配。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of disaccharide recognition by molecularly imprinted polymers.

The selectivity of carbohydrate-imprinted polymers for several disaccharides, namely cellobiose, maltose, lactose and gentiobiose, is investigated. An ternary ligand-Cu(II)-carbohydrate complex was formed in alkaline solution and captured afterwards in the polymer. The accessibility of the polymer matrix for disaccharides was investigated by HPLC analysis, refractometry and 1H NMR spectroscopy applying excess of the original template during rebinding experiments under saturation conditions in unbuffered, aqueous solution at neutral pH and 20 degrees C. The selective discrimination of the alpha- and beta-glycosidic linkage of cellobiose and maltose is demonstrated. It is further shown, that the disaccharide-imprinted polymers slightly distinguish between the 1,4-beta- and the 1,6-beta-glycosidic linkage of cellobiose and gentiobiose, while cellobiose and lactose are not selectively recognized. Due to the weak apparent binding constant of the functional Cu(II) monomers with the targeted disaccharides at physiological pH, the recognition process is dominated by the shape of the created imprinted cavity under the applied conditions.

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