不同疏水配体单步纯化碱性蛋白酶的优化。

Bioseparation Pub Date : 2001-01-01 DOI:10.1023/a:1016313805386

H V Adikane, R S Barhate, D M Thakar, S N Nene

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引用次数: 3

摘要

研究了不同脂肪族和芳香族疏水配体对碱性蛋白酶的吸附和解吸规律。总的来说，配体与6%交联凝胶的吸附比4%凝胶的吸附高。6%交联凝胶对丁基(94%)和苯基(98.4%)的吸附效果最好。吸附作用取决于配体的浓度和性质。在单步操作中，在所有优化的实验参数下，酶的纯度几乎达到20倍，产率达到40%。

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Optimization of single-step purification of alkaline protease using different hydrophobic ligands.

The adsorption and desorption pattern of alkaline protease was studied using different aliphatic and aromatic hydrophobic ligands. Overall, higher adsorption was obtained on ligands coupled to 6% cross-linked gel than the 4% gel. The highest adsorption was obtained on butyl (94%) and phenyl (98.4%) of 6% cross-linked gel. The adsorption was dependent on concentration and nature of the ligand. In a single-step operation, almost 20-fold purification with 40% yield of the enzyme was obtained using all the optimized experimental parameters.

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Bioseparation

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