荧光原位杂交对小鼠、大鼠和仓鼠染色体7个基因的比较定位。

T Ono, S Hirano, S Yonezawa, S Aono, M Osaki, S Masaki, S Yamashita, T Tsukasaki, A Oohira, S T Suzuki, S Sonta
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引用次数: 13

摘要

利用小鼠探针进行荧光原位杂交(FISH),将组织蛋白酶E (Ctse)、原钙粘蛋白10 (Pcdh10,别名OL-protocadherin, Ol-pc)、原钙粘蛋白13 (Pcdh13,别名protocadherin 2c, Pcdh2c)、神经聚糖C (Cspg5)和肌球蛋白X (Myo10)基因的同源物分别定位到大鼠染色体(RNO) 13q13、2q24—>q25、18p12—>p11、8q32.1和2q22.1—>q22.3上。同样,小鼠Ctse、Pcdh13、Cspg5和Myo10基因的同源物以及大鼠Smad2 (Madh2)和Smad4 (Madh4)基因的同源物分别被分配到中国仓鼠染色体(CGR) 5q28、2q17、4q26、2p29- >p27、2q112- >q113和2q112- >q113上。Ctse和Cspg5同源物的染色体分配强化了小鼠染色体(MMU) 1、RNO 13和CGR 5q之间以及小鼠染色体(MMU) 9、RNO 8和CGR 4q之间众所周知的同源关系。Madh2、Madh4和Pcdh13基因同源物的染色体位置表明,在MMU 18和RNO 18的分化过程中,反转事件参与了染色体重排,而MMU 18的大部分在CGR 2q中作为连续片段保存。此外,Myo10及其同源物的定位结果表明mmu15、RNO 2和CGR 2具有同源性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative mapping of seven genes in mouse, rat and Chinese hamster chromosomes by fluorescence in situ hybridization.

By fluorescence in situ hybridization (FISH) using mouse probes, we assigned homologues for cathepsin E (Ctse), protocadherin 10 (Pcdh10, alias OL-protocadherin, Ol-pc), protocadherin 13 (Pcdh13, alias protocadherin 2c, Pcdh2c), neuroglycan C (Cspg5) and myosin X (Myo10) genes to rat chromosomes (RNO) 13q13, 2q24-->q25, 18p12-->p11, 8q32.1 and 2q22.1-->q22.3, respectively. Similarly, homologues for mouse Ctse, Pcdh13, Cspg5 and Myo10 genes and homologues for rat Smad2 (Madh2) and Smad4 (Madh4) genes were assigned to Chinese hamster chromosomes (CGR) 5q28, 2q17, 4q26, 2p29-->p27, 2q112-->q113 and 2q112-->q113, respectively. The chromosome assignments of homologues of Ctse and Cspg5 reinforced well-known homologous relationships among mouse chromosome (MMU) 1, RNO 13 and CGR 5q, and among MMU 9, RNO 8 and CGR 4q, respectively. The chromosome locations of homologues for Madh2, Madh4 and Pcdh13 genes suggested that inversion events were involved in chromosomal rearrangements in the differentiation of MMU 18 and RNO 18, whereas most of MMU 18 is conserved as a continuous segment in CGR 2q. Furthermore, the mapping result of Myo10 and homologues suggested an orthologous segment of MMU 15, RNO 2 and CGR 2.

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