一个进化保守的雄性调控基因DMRT1在鸟类中的性别特异性表达。

Z Shan, I Nanda, Y Wang, M Schmid, A Vortkamp, T Haaf
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引用次数: 102

摘要

基于其z -性染色体位置及其与人类雄性性调节因子(DMRT1和DMRT2)、果蝇(Dsx)和秀丽隐杆线虫(Mab-3)的结构同源性,鸡DMRT1是鸟类睾丸决定因子的绝佳候选者。我们提出的数据为这一假设提供了进一步的有力支持。通过全株原位杂交发现,在胚胎发生早期,DMRT1在鸡雄性生殖器脊中的表达水平高于雌性生殖器脊。它的表达在性别化开始后成为睾丸特异性的。Northern blot和RT - PCR分析显示,DMRT1在成年鸟类中只在睾丸中表达。我们认为,两个基因剂量对Z雄性的睾丸形成是必需的,而在ZW雌性中,单个Z染色体的表达会导致雌性的性别分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sex-specific expression of an evolutionarily conserved male regulatory gene, DMRT1, in birds.

Based on its Z-sex-chromosomal location and its structural homology to male sexual regulatory factors in humans (DMRT1 and DMRT2), Drosophila (Dsx), and Caenorhabditis elegans (Mab-3), chicken DMRT1 is an excellent candidate for a testis-determining factor in birds. The data we present provide further strong support for this hypothesis. By whole mount in situ hybridization chicken DMRT1 is expressed at higher levels in the male than in the female genital ridges during early stages of embryogenesis. Its expression becomes testis-specific after onset of sexual differentiation. Northern blot and RT PCR analysis showed that in adult birds DMRT1 is expressed exclusively in the testis. We propose that two gene dosages are required for testis formation in ZZ males, whereas expression from a single Z chromosome in ZW females leads to female sexual differentiation.

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