猴脑干和脊髓GAT-1阳性细胞的光镜和电镜研究。

Journal fur Hirnforschung Pub Date : 1999-01-01
C H Ng, W Y Ong
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引用次数: 0

摘要

利用一种针对GAT-1 C端合成肽段的亲和纯化多克隆抗体,研究了GABA转运体GAT-1在猴脑干和脊髓中的分布。髓核间核、下橄榄核和脊髓明胶质染色非常致密,黑质、耳蜗核、前庭核、脊髓V核、脊髓后脑区和脊髓前角呈密集标记。电镜显示,标记的图谱由对称突触的轴突末端组成,与gaba能末端一致。许多被密集标记为GAT-1的核是那些接受初级听觉、前庭或体感觉输入的核,这些核中高密度的GAT-1表明GAT-1在终止这些核中GABA的抑制作用方面起着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A light and electron microscopic study of GAT-1 positive cells in the monkey brainstem and spinal cord.

The distribution of the GABA transporter GAT-1 was studied in the monkey brainstem and spinal cord, using an affinity purified polyclonal antibody against a synthetic peptide corresponding to the C terminus of GAT-1. Very dense staining was observed in the interpeduncular nucleus, the inferior olivary nucleus and the substantia gelatinosa of the spinal cord, whilst dense labelling was observed in the substantia nigra, cochlear nuclei, vestibular nuclei, the spinal nucleus of V, the area postrema and the ventral horn of the spinal cord. Electron microscopy showed that the labelled profiles consisted of axon terminals that formed symmetrical synapses, consistent with GABAergic terminals. Many of the nuclei that were densely labelled for GAT-1 were those that received primary auditory, vestibular, or somatosensory inputs and the high density of GAT-1 in these nuclei suggests that GAT-1 plays an important role in terminating the inhibitory effects of GABA, at these nuclei.

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