生物磁分离对人白细胞mRNA的处理:与目前RNA分离方法的比较。

F Mrázek, M Petrek
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引用次数: 0

摘要

本文介绍了利用“Dynabeads mRNA DIRECT”对mRNA进行生物磁分离的技术。在简要介绍了RNA分离方法后,作者描述了BS的一般程序及其在支气管肺泡细胞mRNA分离中的具体应用。作者报告了特定实验的结果,以评估用BS分离mRNA的效率和准确性。文章最后讨论了BS技术在基因表达研究中的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Processing of mRNA from human leukocytes by biomagnetical separation: comparison with current methods of RNA isolation.

The article refers to the technique of biomagnetical separation (BS) of mRNA using "Dynabeads mRNA DIRECT". After a brief survey of RNA isolation methods authors describe a general procedure of BS as well as its concrete application for mRNA isolation from bronchoalveolar cells. Authors report results of specific experiments to assess the efficiency and accuracy of mRNA isolation by BS. The applicability of BS technique for the gene expression studies is discussed in the end of the article.

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