M. Ravikumar , V. Dheenadhayalan , K. Rajaram , S. Shanmuga Lakshmi , P. Paul Kumaran , C.N. Paramasivan , K. Balakrishnan , R.M. Pitchappan
{"title":"HLA-DRB1、DQB1和DPB1等位基因与印度南部肺结核的关系","authors":"M. Ravikumar , V. Dheenadhayalan , K. Rajaram , S. Shanmuga Lakshmi , P. Paul Kumaran , C.N. Paramasivan , K. Balakrishnan , R.M. Pitchappan","doi":"10.1054/tuld.1999.0213","DOIUrl":null,"url":null,"abstract":"<div><p><em>Setting</em>: Tuberculosis is endemic in south India: sputum positive pulmonary tuberculosis is predisposed by HLA-DR2 in south India and few other populations of the world.</p><p><em>Objective</em>: To study HLA-DRB1, DQB1, DQA1 and DPB1 allelic polymorphism in pulmonary tuberculosis patients and endemic controls from south India.</p><p><em>Design</em>: One hundred and twenty-six, sputum positive pulmonary tuberculosis patients and 87, endemic controls, from Madurai were studied for MHC class II allelic polymorphism by PCR-SSOP method. XI IHWC primers and probes and non-radioactive probing methods were employed.</p><p><em>Results</em>: HLA DRB1*1501 and DQB1*0601 predisposed for pulmonary tuberculosis (DRB1*1501: odds ratio (OR)=2.68, 95% confidence interval (CI)=1.30–5.89, <em>P</em> value (<em>P</em>)=0.013, aetiological fraction (EF)=0.17; DQB1*0601: OR=2.32, CI=1.29–4.27, <em>P</em>=0.008, EF=0.26). Haplotype DRB1*1501–DQB1*0601 was higher in patients (1324 per 10000, X<sup>2</sup>=27.07) than controls (F=404÷10000, X<sup>2</sup>= 8.84). In a subset of 63 caste matched samples, DPB1*04 was preventive (OR=0.45, CI=0.21-0.95, <em>P</em>=0.036, PF=0.26): the distributions of DRB1*1501-DQB1*0601-DPB1*04 phenotypes were different between patients and controls (<em>P</em>=0.0092). These alleles were predominant in patients and controls of T5SU caste.</p><p><em>Conclusion</em>: HLA-DRB1*1501 and DQB1*0601 predisposed to sputum positive pulmonary tuberculosis, and DPB1*04 was preventive and epistatic to this risk. Caste T5SU is an ideal model to study immunology of tuberculosis.</p></div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"79 5","pages":"Pages 309-317"},"PeriodicalIF":0.0000,"publicationDate":"1999-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.1999.0213","citationCount":"158","resultStr":"{\"title\":\"Associations of HLA-DRB1, DQB1 and DPB1 alleles with pulmonary tuberculosis in south India\",\"authors\":\"M. Ravikumar , V. Dheenadhayalan , K. Rajaram , S. Shanmuga Lakshmi , P. Paul Kumaran , C.N. Paramasivan , K. Balakrishnan , R.M. Pitchappan\",\"doi\":\"10.1054/tuld.1999.0213\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Setting</em>: Tuberculosis is endemic in south India: sputum positive pulmonary tuberculosis is predisposed by HLA-DR2 in south India and few other populations of the world.</p><p><em>Objective</em>: To study HLA-DRB1, DQB1, DQA1 and DPB1 allelic polymorphism in pulmonary tuberculosis patients and endemic controls from south India.</p><p><em>Design</em>: One hundred and twenty-six, sputum positive pulmonary tuberculosis patients and 87, endemic controls, from Madurai were studied for MHC class II allelic polymorphism by PCR-SSOP method. XI IHWC primers and probes and non-radioactive probing methods were employed.</p><p><em>Results</em>: HLA DRB1*1501 and DQB1*0601 predisposed for pulmonary tuberculosis (DRB1*1501: odds ratio (OR)=2.68, 95% confidence interval (CI)=1.30–5.89, <em>P</em> value (<em>P</em>)=0.013, aetiological fraction (EF)=0.17; DQB1*0601: OR=2.32, CI=1.29–4.27, <em>P</em>=0.008, EF=0.26). Haplotype DRB1*1501–DQB1*0601 was higher in patients (1324 per 10000, X<sup>2</sup>=27.07) than controls (F=404÷10000, X<sup>2</sup>= 8.84). In a subset of 63 caste matched samples, DPB1*04 was preventive (OR=0.45, CI=0.21-0.95, <em>P</em>=0.036, PF=0.26): the distributions of DRB1*1501-DQB1*0601-DPB1*04 phenotypes were different between patients and controls (<em>P</em>=0.0092). These alleles were predominant in patients and controls of T5SU caste.</p><p><em>Conclusion</em>: HLA-DRB1*1501 and DQB1*0601 predisposed to sputum positive pulmonary tuberculosis, and DPB1*04 was preventive and epistatic to this risk. Caste T5SU is an ideal model to study immunology of tuberculosis.</p></div>\",\"PeriodicalId\":77450,\"journal\":{\"name\":\"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease\",\"volume\":\"79 5\",\"pages\":\"Pages 309-317\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1054/tuld.1999.0213\",\"citationCount\":\"158\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0962847999902137\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0962847999902137","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Associations of HLA-DRB1, DQB1 and DPB1 alleles with pulmonary tuberculosis in south India
Setting: Tuberculosis is endemic in south India: sputum positive pulmonary tuberculosis is predisposed by HLA-DR2 in south India and few other populations of the world.
Objective: To study HLA-DRB1, DQB1, DQA1 and DPB1 allelic polymorphism in pulmonary tuberculosis patients and endemic controls from south India.
Design: One hundred and twenty-six, sputum positive pulmonary tuberculosis patients and 87, endemic controls, from Madurai were studied for MHC class II allelic polymorphism by PCR-SSOP method. XI IHWC primers and probes and non-radioactive probing methods were employed.
Results: HLA DRB1*1501 and DQB1*0601 predisposed for pulmonary tuberculosis (DRB1*1501: odds ratio (OR)=2.68, 95% confidence interval (CI)=1.30–5.89, P value (P)=0.013, aetiological fraction (EF)=0.17; DQB1*0601: OR=2.32, CI=1.29–4.27, P=0.008, EF=0.26). Haplotype DRB1*1501–DQB1*0601 was higher in patients (1324 per 10000, X2=27.07) than controls (F=404÷10000, X2= 8.84). In a subset of 63 caste matched samples, DPB1*04 was preventive (OR=0.45, CI=0.21-0.95, P=0.036, PF=0.26): the distributions of DRB1*1501-DQB1*0601-DPB1*04 phenotypes were different between patients and controls (P=0.0092). These alleles were predominant in patients and controls of T5SU caste.
Conclusion: HLA-DRB1*1501 and DQB1*0601 predisposed to sputum positive pulmonary tuberculosis, and DPB1*04 was preventive and epistatic to this risk. Caste T5SU is an ideal model to study immunology of tuberculosis.
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