{"title":"海洋鱼类光斑Sparus aurata胚胎干细胞的研究多传代维持、鉴定和转染","authors":"J. Béjar , Y. Hong , M.C. Alvarez","doi":"10.1016/S1050-3862(99)00015-7","DOIUrl":null,"url":null,"abstract":"<div><p>Animal Embryonic-Stem (ES) cells represents a unique tool in animal genetic manipulation. Though putative ES cells from several species have been reported, only those from mice proved successful. In this work, a long-term embryonic cell culture, derived from the commercial fish (<em>Sparus aurata</em>), is reported. These cells have been in vitro characterized for totipotency and transfected with a GFP plasmid.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 3","pages":"Pages 125-129"},"PeriodicalIF":0.0000,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00015-7","citationCount":"23","resultStr":"{\"title\":\"Towards obtaining ES cells in the marine fish species Sparus aurata; multipassage maintenance, characterization and transfection\",\"authors\":\"J. Béjar , Y. Hong , M.C. Alvarez\",\"doi\":\"10.1016/S1050-3862(99)00015-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Animal Embryonic-Stem (ES) cells represents a unique tool in animal genetic manipulation. Though putative ES cells from several species have been reported, only those from mice proved successful. In this work, a long-term embryonic cell culture, derived from the commercial fish (<em>Sparus aurata</em>), is reported. These cells have been in vitro characterized for totipotency and transfected with a GFP plasmid.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"15 3\",\"pages\":\"Pages 125-129\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00015-7\",\"citationCount\":\"23\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386299000157\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386299000157","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Towards obtaining ES cells in the marine fish species Sparus aurata; multipassage maintenance, characterization and transfection
Animal Embryonic-Stem (ES) cells represents a unique tool in animal genetic manipulation. Though putative ES cells from several species have been reported, only those from mice proved successful. In this work, a long-term embryonic cell culture, derived from the commercial fish (Sparus aurata), is reported. These cells have been in vitro characterized for totipotency and transfected with a GFP plasmid.
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