{"title":"真核启动子在水产养殖DNA疫苗构建中的应用评价","authors":"Marta Gómez-Chiarri, Laurie A Chiaverini","doi":"10.1016/S1050-3862(99)00014-5","DOIUrl":null,"url":null,"abstract":"<div><p>We evaluated fish promoters as an alternative to viral promoters in the construction of DNA vaccines for aquaculture. A carp β-actin promoter drove expression of the luciferase gene in live fish tissue to levels comparable to the CMVtk promoter.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"15 3","pages":"Pages 121-124"},"PeriodicalIF":0.0000,"publicationDate":"1999-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00014-5","citationCount":"23","resultStr":"{\"title\":\"Evaluation of eukaryotic promoters for the construction of DNA vaccines for aquaculture\",\"authors\":\"Marta Gómez-Chiarri, Laurie A Chiaverini\",\"doi\":\"10.1016/S1050-3862(99)00014-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We evaluated fish promoters as an alternative to viral promoters in the construction of DNA vaccines for aquaculture. A carp β-actin promoter drove expression of the luciferase gene in live fish tissue to levels comparable to the CMVtk promoter.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"15 3\",\"pages\":\"Pages 121-124\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(99)00014-5\",\"citationCount\":\"23\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386299000145\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386299000145","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of eukaryotic promoters for the construction of DNA vaccines for aquaculture
We evaluated fish promoters as an alternative to viral promoters in the construction of DNA vaccines for aquaculture. A carp β-actin promoter drove expression of the luciferase gene in live fish tissue to levels comparable to the CMVtk promoter.
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