NMDA和非NMDA受体参与猫脊髓内脏痛觉的传递。

X J Song, Z Q Zhao
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引用次数: 0

摘要

目的:研究n-甲基- d -天冬氨酸(NMDA)和非NMDA受体在脊髓损伤性内脏信息加工中的作用。方法:记录25只麻醉猫脊髓背角神经元经乳胶球囊充气至结直肠膨胀(3 ~ 15 kPa, 20 s)的放电情况。结果:1)根据对结肠膨胀的反应模式,放电增加和减少的神经元分别属于兴奋性和抑制性。前者由17个短潜伏期突变(SLA)神经元、11个短潜伏期持续(SLS)神经元和9个长潜伏期(LL)神经元组成。记录15个抑制神经元(Inh)。2) NMDA、准qualic acid (QA)和kainic acid (KA)的微电泳激活了67.6%、78.4%和59.5%的结肠膨胀兴奋神经元。分别有60%、86.7%和53.3%的Inh神经元被这3种氨基酸激活。3)选择性NMDA受体拮抗剂d,l-2-氨基-5-磷酸戊酸酯(APV)和选择性非NMDA受体拮抗剂6,7-二硝基-喹啉-2,3-二酮(DNQX)分别使结直肠膨胀诱导的兴奋反应降低35% +/- 10%和65% +/- 14%。dnqx诱导的抑制作用明显强于APV (P < 0.05)。DNQX在3/7 Inh神经元中部分缓解结肠膨胀诱导的抑制反应30%-50%,而APV则没有。结论:NMDA和非NMDA受体均参与脊髓内脏伤害性信息的传递和/或调节,非NMDA受体可能比NMDA受体发挥更重要的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of NMDA and non-NMDA receptors in transmission of spinal visceral nociception in cat.

Aim: To study the role of N-methyl-D-aspartic acid (NMDA) and non-NMDA receptors in processing nociceptive visceral information in the spinal cord.

Methods: The firing of spinal dorsal horn neurons to colorectal distension (3-15 kPa, 20 s) by inflation with air of latex balloon was recorded in 25 anesthetized cats.

Results: 1) According to the patterns of responses to colorectal distension, the neurons with increase and decrease in firing were classified as excitatory and inhibitory, respectively. The former consisted of 17 short-latency abrupt (SLA) neurons, 11 short-latency sustained (SLS) neurons, 9 long-latency (LL) neurons. The 15 inhibited (Inh) neurons were recorded. 2) Microelectrophoretic administration of NMDA, quisqualic acid (QA), and kainic acid (KA) activated 67.6%, 78.4%, and 59.5% of the colorectal distension-excited neurons tested. Also, 60%, 86.7%, and 53.3% of Inh neurons were activated by these 3 amino acids. 3) Colorectal distension-induced excitatory responses were reduced by 35% +/- 10% and 65% +/- 14% by a selective NMDA receptor antagonist d,l-2-amino-5-phosphonovalerate (APV) and a selective non-NMDA receptor antagonist 6,7-dinitro-quinoxaline-2,3-dione (DNQX), respectively. Such DNQX-induced inhibition was significantly more potent than that by APV (P < 0.05). Colorectal distension-induced inhibitory responses were partially relieved by 30%-50% in 3/7 Inh neurons by DNQX, but not APV.

Conclusion: Both NMDA and non-NMDA receptors are involved in transmission and/or modulation of spinal visceral nociceptive information and non-NMDA receptors may play more important role than NMDA receptors.

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