小檗碱对KCl、去甲肾上腺素和咖啡因对新生大鼠心肌细胞[Ca2+]i动员的拮抗作用。

G F Qiao, H Zhou, B Y Li, W H Li
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引用次数: 0

摘要

目的:研究小檗碱(Ber)对KCl去极化、去甲肾上腺素(NE)和咖啡因动员的细胞内钙离子浓度([Ca2+]i)的影响。方法:在共聚焦显微镜下,用荧光强度(FI)测定负载Fluo 3-AM 2 mumol.L-1的新生大鼠单培养心肌细胞中的[Ca2+]i。结果:在细胞外钙1.5 mmol的情况下,对照组[Ca2+]i的FI值为248 +/- 70。L-1,未被3-30 momol .L-1改变。Ber 30 mumol可抑制KCl (60 mmol.L-1)-和NE (30 mmol.L-1)诱导的[Ca2+]i动员(P < 0.01)。L-1,与维拉帕米(Ver)相似。在3 mmol乙酸的存在下,Ber对KCl诱导的[Ca2+]i的抑制作用进一步增强(P < 0.05)。而NE对[Ca2+]i的影响没有变化。[Ca2+]被咖啡因80和160 mumol调动。Ber和Ver对D-Hanks液中L-1的影响不显著(P > 0.05)。结论:Ber通过电压依赖性Ca2+通道和受体操作的Ca2+通道对新生大鼠心肌细胞[Ca2+]i的增加具有拮抗作用,但对细胞内Ca2+释放无影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antagonistic effects of berbamine on [Ca2+]i mobilization by KCl, norepinephrine, and caffeine in newborn rat cardiomyocytes.

Aim: To study the effects of berbamine (Ber) on intracellular calcium concentration ([Ca2+]i) mobilized by KCl depolarization, norepinephrine (NE), and caffeine.

Methods: [Ca2+]i was measured with fluorescent intensity (FI) by confocal microscope in single cultured cardiomyocytes of newborn rats loaded with Fluo 3-AM 2 mumol.L-1.

Results: FI value of [Ca2+]i in control level was 248 +/- 70 in the presence of extracellular calcium 1.5 mmol.L-1 and was not changed by Ber 3-30 mumol.L-1. KCl (60 mmol.L-1)- and NE (30 mumol.L-1)-induced [Ca2+]i mobilizations were inhibited (P < 0.01) by Ber 30 mumol.L-1, similar to that of verapamil (Ver). The inhibitory effect of Ber on [Ca2+]i induced by KCl was further increased (P < 0.05) in the presence of egtazic acid 3 mmol.L-1, but that on [Ca2+]i induced by NE was not changed. The [Ca2+]i mobilized by caffeine 80 and 160 mumol.L-1 in D-Hanks' solution was not affected (P > 0.05) by Ber and Ver.

Conclusion: Ber possessed the antagonistic effects on [Ca2+]i increases via voltage-dependent Ca2+ channel and receptor-operated Ca2+ channel in newborn rat cardiomyocytes, but without effect on intracellular Ca2+ release.

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