M D Campos-Almaraz, O Barbosa-Cisneros, R Herrera-Esparza
{"title":"Ro60核糖核蛋白在体外通过T3 RNA聚合酶抑制转录。","authors":"M D Campos-Almaraz, O Barbosa-Cisneros, R Herrera-Esparza","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Ro60 ribonucleoprotein is a conserved molecule belonging to the family of Ro ribonucleoproteins and targeted by autoantibodies produced in patients with systemic lupus erythematosus or Sjogren's syndrome. Ro60 plays a role in postranscription events, as well as in the nucleocytoplasmic shuttling of RNA polymerase III transcripts.</p><p><strong>Objective: </strong>To evaluate the in vitro effects of Ro60 on T3 RNA polymerase transcription.</p><p><strong>Methods: </strong>Ro60 ribonucleoprotein was affinity-purified from human spleen extracts using 4B-Sepharose linked to anti-Ro60 monoclonal antibodies. Purified Ro60 was incorporated into the T3 RNA polymerase transcription reaction system using pTRI-beta-Actin-human DNA as a template.</p><p><strong>Results: </strong>Ro60 inhibited initiation of the transcription process in a dose-dependent manner; neither elongation nor termination was affected by Ro.</p><p><strong>Conclusion: </strong>In vitro, Ro60 appears to inhibit the transcription of a T3 RNA polymerase-dependent template.</p>","PeriodicalId":79371,"journal":{"name":"Revue du rhumatisme (English ed.)","volume":"66 6","pages":"310-4"},"PeriodicalIF":0.0000,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ro60 ribonucleoprotein inhibits transcription by T3 RNA polymerase in vitro.\",\"authors\":\"M D Campos-Almaraz, O Barbosa-Cisneros, R Herrera-Esparza\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Ro60 ribonucleoprotein is a conserved molecule belonging to the family of Ro ribonucleoproteins and targeted by autoantibodies produced in patients with systemic lupus erythematosus or Sjogren's syndrome. Ro60 plays a role in postranscription events, as well as in the nucleocytoplasmic shuttling of RNA polymerase III transcripts.</p><p><strong>Objective: </strong>To evaluate the in vitro effects of Ro60 on T3 RNA polymerase transcription.</p><p><strong>Methods: </strong>Ro60 ribonucleoprotein was affinity-purified from human spleen extracts using 4B-Sepharose linked to anti-Ro60 monoclonal antibodies. Purified Ro60 was incorporated into the T3 RNA polymerase transcription reaction system using pTRI-beta-Actin-human DNA as a template.</p><p><strong>Results: </strong>Ro60 inhibited initiation of the transcription process in a dose-dependent manner; neither elongation nor termination was affected by Ro.</p><p><strong>Conclusion: </strong>In vitro, Ro60 appears to inhibit the transcription of a T3 RNA polymerase-dependent template.</p>\",\"PeriodicalId\":79371,\"journal\":{\"name\":\"Revue du rhumatisme (English ed.)\",\"volume\":\"66 6\",\"pages\":\"310-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revue du rhumatisme (English ed.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revue du rhumatisme (English ed.)","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Ro60 ribonucleoprotein inhibits transcription by T3 RNA polymerase in vitro.
Background: Ro60 ribonucleoprotein is a conserved molecule belonging to the family of Ro ribonucleoproteins and targeted by autoantibodies produced in patients with systemic lupus erythematosus or Sjogren's syndrome. Ro60 plays a role in postranscription events, as well as in the nucleocytoplasmic shuttling of RNA polymerase III transcripts.
Objective: To evaluate the in vitro effects of Ro60 on T3 RNA polymerase transcription.
Methods: Ro60 ribonucleoprotein was affinity-purified from human spleen extracts using 4B-Sepharose linked to anti-Ro60 monoclonal antibodies. Purified Ro60 was incorporated into the T3 RNA polymerase transcription reaction system using pTRI-beta-Actin-human DNA as a template.
Results: Ro60 inhibited initiation of the transcription process in a dose-dependent manner; neither elongation nor termination was affected by Ro.
Conclusion: In vitro, Ro60 appears to inhibit the transcription of a T3 RNA polymerase-dependent template.
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