K Haghjoo, P W Cash, R S Farid, B R Komisaruk, F Jordan, S S Pochapsky
{"title":"具有镇痛作用的血管活性肠多肽(11-28)-NH2的溶液结构。","authors":"K Haghjoo, P W Cash, R S Farid, B R Komisaruk, F Jordan, S S Pochapsky","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>An 18-residue-long fragment of vasoactive intestinal polypeptide [VIP(11-28)-NH2] that is known to be analgesic was synthesized by solid-phase t-Boc methodology on a 4-methylbenzhydrylamine resin. Circular dichroism spectroscopy gave evidence that the peptid acquires about 60% helical structure in 50/50 methanol/phosphate buffer, pH 6.0, and 65% (+/-5%) helicity in 80/20 methanol/phosphate buffer pH 7.0, A 2.0 mM solution of VIP (11-28) NH2 in 80% methanol, 20% phosphate buffer pH 7.0 was subjected to 2-dimensional nuclear magnetic resonance (NMR) studies The NMR results suggested formation of an extended helical structure extending from residue 11 to 27 essentially the same region found to be helical in a VIP(1-28)-NH2 and log. This finding suggests that the sequence required for analgesia assumes a helical structure at the receptor.</p>","PeriodicalId":20005,"journal":{"name":"Peptide research","volume":"9 6","pages":"327-31"},"PeriodicalIF":0.0000,"publicationDate":"1996-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Solution structure of vasoactive intestinal polypeptide (11-28)-NH2, a fragment with analgesic properties.\",\"authors\":\"K Haghjoo, P W Cash, R S Farid, B R Komisaruk, F Jordan, S S Pochapsky\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An 18-residue-long fragment of vasoactive intestinal polypeptide [VIP(11-28)-NH2] that is known to be analgesic was synthesized by solid-phase t-Boc methodology on a 4-methylbenzhydrylamine resin. Circular dichroism spectroscopy gave evidence that the peptid acquires about 60% helical structure in 50/50 methanol/phosphate buffer, pH 6.0, and 65% (+/-5%) helicity in 80/20 methanol/phosphate buffer pH 7.0, A 2.0 mM solution of VIP (11-28) NH2 in 80% methanol, 20% phosphate buffer pH 7.0 was subjected to 2-dimensional nuclear magnetic resonance (NMR) studies The NMR results suggested formation of an extended helical structure extending from residue 11 to 27 essentially the same region found to be helical in a VIP(1-28)-NH2 and log. This finding suggests that the sequence required for analgesia assumes a helical structure at the receptor.</p>\",\"PeriodicalId\":20005,\"journal\":{\"name\":\"Peptide research\",\"volume\":\"9 6\",\"pages\":\"327-31\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Peptide research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Peptide research","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用固相t-Boc方法在4-甲基苯基胺树脂上合成了一个18个残基长的血管活性肠多肽[VIP(11-28)-NH2]片段,该片段已知具有镇痛作用。圆二色光谱表明,肽在50/50甲醇/磷酸盐缓冲液中,pH值为6.0,获得约60%的螺旋结构,在80/20甲醇/磷酸盐缓冲液中,pH值为7.0,VIP (11-28) NH2溶液为2.0 mM, 80%甲醇中,获得65%(+/-5%)的螺旋结构。对pH 7.0的20%磷酸盐缓冲液进行了二维核磁共振(NMR)研究。核磁共振结果表明,从残基11到残基27形成了一个延伸的螺旋结构,与VIP(1-28)-NH2和log中发现的螺旋结构基本相同。这一发现表明,镇痛所需的序列在受体处呈螺旋结构。
Solution structure of vasoactive intestinal polypeptide (11-28)-NH2, a fragment with analgesic properties.
An 18-residue-long fragment of vasoactive intestinal polypeptide [VIP(11-28)-NH2] that is known to be analgesic was synthesized by solid-phase t-Boc methodology on a 4-methylbenzhydrylamine resin. Circular dichroism spectroscopy gave evidence that the peptid acquires about 60% helical structure in 50/50 methanol/phosphate buffer, pH 6.0, and 65% (+/-5%) helicity in 80/20 methanol/phosphate buffer pH 7.0, A 2.0 mM solution of VIP (11-28) NH2 in 80% methanol, 20% phosphate buffer pH 7.0 was subjected to 2-dimensional nuclear magnetic resonance (NMR) studies The NMR results suggested formation of an extended helical structure extending from residue 11 to 27 essentially the same region found to be helical in a VIP(1-28)-NH2 and log. This finding suggests that the sequence required for analgesia assumes a helical structure at the receptor.