聚合酶链反应在眼弓形虫病诊断中的局限性。

German journal of ophthalmology Pub Date : 1996-03-01
J Garweg, M Boehnke, F Koerner
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引用次数: 0

摘要

据报道,通过前房水龙头的聚合酶链反应(PCR)技术可以促进眼弓形虫病的实验室确认。我们利用PCR技术对刚地弓形虫b1基因序列进行了特异性分析,该序列长度为194 bp。在染色凝胶中,灵敏度调整为每个样品10个基因组拷贝,使用二氧标记探针进行DNA杂交后,灵敏度调整为2个拷贝。我们从连续31例临床诊断为眼弓形虫病的健康患者中提取了43份水样、14份血清样和32份白细胞(WBC)样本,扩增了DNA。在调查的系列中,1/43的水样和2/32的白细胞样被发现含有可检测量的目标DNA。排除了房水抑制PCR作为低检出率的原因。因此,我们得出结论,房水PCR的敏感性不足以用于免疫正常个体的眼部弓形虫病的常规诊断,或者前房不是使用这种方法调查这种原生动物疾病的适当隔间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Restricted applicability of the polymerase chain reaction for the diagnosis of ocular toxoplasmosis.

The laboratory confirmation of ocular toxoplasmosis has been reported to be facilitated by the polymerase chain reaction (PCR) technique in anterior chamber taps. We utilized PCR specific for a sequence of the Toxoplasma gondii B 1 gene with a length of 194 bp. The sensitivity was adjusted to ten genomic copies per sample in stained gel and two copies after DNA hybridization using a digoxygenin-labeled probe. We amplified DNA from 43 aqueous, 14 serum, and 32 white blood cell (WBC) samples obtained from 31 consecutive otherwise healthy patients with the clinical diagnosis of ocular toxoplasmosis. In the series investigated, 1/43 aqueous samples and 2/32 WBC samples were found to contain detectable amounts of target DNA. An inhibition of the PCR by the aqueous humor as a reason for the low detection rates was excluded. Thus, we conclude that either the sensitivity of aqueous humor PCR is not sufficient for use in the routine diagnosis of ocular toxoplasmosis in immuno-competent individuals or the anterior chamber is not the proper compartment for investigation of this protozoal disease using this approach.

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