Ahmed F. Abdel-Fattah , Abdel-Mohsen S. Ismail , Soad A. Saleh
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引用次数: 1
摘要
酵母浸膏与蛋白胨混合的培养基最有利于尖孢镰刀菌N.R.C.1产生活性纤溶酶。磷酸二氢钾对酶产率有促进作用,而葡萄糖、蔗糖、乳糖、核糖和可溶性淀粉对酶产率有不利影响。纤维蛋白溶酶活性在pH 7.0时达到最佳。在培养培养基中提取纤维蛋白水解酶与蛋白胨的关系及其在尖孢镰刀菌N.R.C.l中活性纤维蛋白水解酶的产生。磷酸二氢钾刺激,während葡萄糖,糖,乳糖,核糖和lösliche Stärke酶产生阴性影响ß 10。Das纤溶酶zigte优化Wirkung bei einem pH-Wert von 7.0。
Production and some properties of fibrinolytic enzymes from Fusarium oxysporum N.R.C.1
A mixture of yeast extract and peptone in the culture medium was the most favourable for the production of active fibrinolytic enzyme by Fusarium oxysporum N.R.C.1. Potassium dihydrogen phosphate had stimulating effect, while glucose, sucrose, lactose, ribose and soluble starch had adverse effect on enzyme productivity. The optimum of the fibrinolytic enzyme activity was at pH 7.0.
Ein Kulturmedium mit Hefeextrakt und Pepton war für die Produktion von aktivem fibrinolytischen Enzym mit Fusarium oxysporum N.R.C.l am besten geeignet. Kaliumdihydrogenphosphat hatte stimulierende Wirkung, während Glucose, Saccharose, Lactose, Ribose und lösliche Stärke die Enzymproduktion negativ beeinflußten. Das fibrinolytische Enzym zeigte optimale Wirkung bei einem pH-Wert von 7.0.