125I标记t - acttemulin (TAM)的药代动力学研究。

I Petkova, O Koparanova, Z Kemileva, S Milanov
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引用次数: 0

摘要

本研究的目的是用放射性碘标记TAM,并跟踪其在大鼠不同器官中的分布。t -动调素是一种新技术从小牛胸腺中分离得到的胸腺激素。TAM按照Greenwood和Hunter的方法进行标记。凝胶层析上观察到两个主要峰:第一个峰含有标记的TAM;第二个峰含有125I,它不与有机化合物结合。在Sephadex G-75柱上对第一个峰进行额外纯化,洗脱液被指定为纯化的峰I。经过放射免疫测试,可以得出结论,TAM被成功标记,峰I含有125I-TAM。峰I、纯化峰I和峰II分别应用于年轻雄性Wistar大鼠。注射后10 min、60 min、24 h处死动物,分别测定胰腺、肾脏、脾脏、肾上腺、肝脏、胸腺、骨髓、睾丸、甲状腺的放射性。药代动力学研究表明,除甲状腺外,所有器官对标记TAM的亲和力高于游离125I。标记TAM在胰腺、肾脏、肾上腺和脾脏中积聚较多。提示这些器官对标记TAM有特异性摄取。讨论了胸腺与胰腺、脾脏、肾上腺的功能关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pharmacokinetic investigation with 125I labeled T-actemodulin (TAM).

The aim of the study was to label TAM with radioactive iodine and to follow its distribution in different organs of rats. T-actemodulin is a thymic hormone isolated from calf thymus by a new technology. TAM was labeled according to the method of Greenwood and Hunter. Two major peaks were observed on the gel chromatography: The first peak contained the labeled TAM; the second peak contained 125I which was not bound to the organic compound. Additional purification of the first peak was performed on a Sephadex G-75 column, and the eluate was designated as purified peak I. After a radioimmune testing it could be concluded that TAM was labeled successfully and the peak I contained 125I-TAM. Peak I, purified peak I and the peak II were applied to young male Wistar rats. The radioactivity of pancreas, kidney, spleen, adrenals, liver, thymus, bone marrow, testis and thyroid were measured after killing the animals 10 min, 60 min, and 24 h after the injections. The pharmacokinetic study showed that all organs, except for the thyroid have a higher affinity for labeled TAM than for the free 125I. Higher accumulation of labeled TAM was observed in the pancreas, kidney, adrenals and spleen. A specific uptake of labeled TAM in these organs can be suggested. Functional interrelations between thymus and pancreas, spleen, adrenals are discussed.

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