人血清生长激素结合蛋白与IM-9淋巴细胞释放的水溶性生长激素结合位点的比较。

G Massa, M Ilondo, M Vanderschueren-Lodeweyckx
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引用次数: 10

摘要

比较了人血清生长激素结合蛋白(GHBP)与培养的IM-9淋巴细胞在25 mmol/l碘乙酰胺缓冲液中培养的水溶性gh结合位点的特性。用125I标记的人生长激素([125I]hGH)培养的水溶性GH结合位点进行高效液相色谱凝胶过滤,发现在血清中与GHBP结合的[125I]hGH的峰值相同的位置有一个大的结合[125I]hGH的峰值。峰的估计M(r)为120,000,可能代表一个[125I]hGH结合到两个结合位点。血清GHBP、水溶性GH结合位点和IM-9淋巴细胞上GH受体的结合特异性相同。血清GHBP对22,000 hGH和20,000 hGH的结合亲和力与水溶性GH结合位点的结合亲和力相似,但低于细胞GH受体的结合亲和力。这些发现表明,血清GHBP的特征与IM-9细胞释放的水溶性GH结合位点相似,并支持了人类血清GHBP是通过细胞GH受体的蛋白水解裂解产生的假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison between the human serum growth hormone-binding protein and the water-soluble growth hormone-binding site released from IM-9 lymphocytes.

The characteristics of the human serum growth hormone-binding protein (GHBP) were compared with those of a water-soluble GH-binding site prepared by incubating cultured IM-9 lymphocytes in assay buffer with 25 mmol/l iodoacetamide. High-performance liquid chromatography gel filtration of the water-soluble GH-binding site incubated with 125I-labeled human GH ([125I]hGH) revealed a large peak of bound [125I]hGH eluting at the same position as the peak of [125I]hGH bound to the GHBP in serum. The estimated M(r) of the peak was 120,000, presumably representing one [125I]hGH bound to two binding sites. The binding specificities of the serum GHBP, the water-soluble GH-binding site and the GH receptor on IM-9 lymphocytes were identical. The binding affinities for 22,000 hGH and for 20,000 hGH of the serum GHBP were similar to the binding affinity of the water-soluble GH-binding site but lower than those of the cellular GH receptor. These findings show that the characteristics of the serum GHBP are comparable to those of the water-soluble GH-binding site released from IM-9 cells and support the hypothesis that in man the serum GHBP is produced by proteolytic cleavage of the cellular GH receptor.

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