低浓度甲状旁腺激素对牛肾皮质膜腺苷酸环化酶的激活作用。

E B Heath, J E Zull
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引用次数: 7

摘要

在减少甲状旁腺激素(PTH)非特异性吸附的条件下,肾膜腺苷酸环化酶对PTH的敏感性显著增强。在低至2 × 10-11M的激素浓度下,可以观察到酶的刺激。此外,激素激活的动力学分析表明,在非特异性吸附较大的条件下,edie - hofstee图向下凹,而当这种吸附减少时,这些图向上凹。这些结果表明,在没有非特异性吸附的情况下,PTH激活肾膜腺苷酸环化酶可以在激素浓度接近生理水平时发生。此外,在低激素浓度下,甲状旁腺激素的激活可能比以前认识到的更复杂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Activation of bovine renal cortex membrane adenylyl cyclase by low concentrations of parathyroid hormone.

Under conditions designed to reduce non-specific adsorption of parathyroid hormone (PTH), the sensitivity of the renal membrane adenylyl cyclase to PTH was significantly enhanced. Stimulation of the enzyme could be observed at hormone concentrations as low as 2 x 10-11M. In addition, kinetic analysis of hormone activation revealed that under conditions where non-specific adsorption is great, downward concavity of Eadie-Hofstee plots is observed, whereas when such adsorption is reduced these plots become upwardly concave. These results suggest that in the absence of non-specific adsorption, PTH activation of kidney membrane adenylyl cyclase can occur at hormone concentrations approaching physiological. In addition, at low hormone concentrations PTH activation is probably more complex than previously recognized.

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