锌离子(Zn2+)对PMN白细胞促凝活性的影响。

E Gazdy, H Csernyánszky, T Szilágyi
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引用次数: 0

摘要

研究了Zn2+、Mg2+和Mn2+对内毒素处理家兔腹膜PMN白细胞促凝活性的影响。从腹腔中收集的细胞在汉克氏溶液中与这些二价阳离子在37度下孵育,然后在柠檬酸处理的兔血浆中测定其上清的凝血活性。当细胞在50 μ m /l ZnCl2中孵育时,其上清液的促凝活性降低了30%。应用100微米/升的ZnCl2后,观察到凝固时间增加了50-60%。锌离子以浓度依赖的方式抑制促凝物质从白细胞的释放。如果在类似的系统中测定,作为对照的其他两种阳离子都不会影响凝血时间。锌离子抑制内毒素处理的PMN白细胞中组织因子的释放。抑制作用呈剂量依赖性,从5 μ m /l呈指数增加到250 μ m /l。锌离子对组织因子释放的抑制作用可能与膜稳定性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of zinc ions (Zn2+) on the procoagulant activity of PMN leukocytes.

The effects of Zn2+, Mg2+ and Mn2+ in vitro were studied on the procoagulant activity of peritoneal PMN leukocytes derived from endotoxin treated rabbits. The cells collected from the abdominal cavity were incubated in the presence of these divalent cations at 37 degrees in Hank's solution followed by determination of the clotting activity of their supernatants in citrate treated rabbit plasma. When the cells were incubated in the presence of 50 microM/l ZnCl2 the procoagulant activity of their supernatants decreased by 30%. Upon the application of 100 microM/l ZnCl2 a 50-60% increase in clotting time was observed. Zinc ions inhibited in a concentration dependent manner the release of procoagulant material from leukocytes. Neither of the other cations used as controls has influenced clotting time if assayed in a similar system. It has been established that zinc ions inhibited the liberation of tissue factor from endotoxin treated PMN leukocytes. The inhibitory effect was dose dependent and increased from 5 microM/l to 250 microM/l exponentially. The inhibitory effect of zinc ions on tissue factor liberation might be explained by membrane stabilization.

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