人脑肌球蛋白的分离、性质及P含量。

S Fazekas, I Ováry, E Horváth, V Székessy-Hermann, P Juhász
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引用次数: 0

摘要

从中枢神经系统不同部位制备KCl-肌球蛋白和nacl -肌球蛋白。在整个实验过程中,nacl -肌球蛋白的P和脂质含量高于kcl -制剂。与骨骼肌肌球蛋白相比,KCl-肌球蛋白和nacl -肌球蛋白均增加了脂质和P含量。用分子筛分离样品时,在Sepharose 4B色谱柱上发现脑肌球蛋白和小脑肌球蛋白由不同分子质量的两个部分组成,而脑干肌球蛋白和脊髓肌球蛋白仅呈现单峰。兔肌动蛋白可提高肌球蛋白组分ca - atp酶活性。肌球蛋白的制备形成丝状系统和聚集体,可通过扫描电镜观察。所有cns -肌球蛋白制剂均可磷酸化;然而,它们的饱和程度不同,并受到血清素存在与否的影响。动力学研究表明,脑干、小脑和脑肌球蛋白的磷酸盐饱和度与ATP浓度和孵育时间有关。无脂人脑肌球蛋白制剂的碱性水解产物中含有不同数量的氨基酸磷酸盐、p -精氨酸、p -赖氨酸和P-His。在磷酸化混合物的作用下,只有脑肌球蛋白中p -精氨酸的含量升高,脑干制剂中p -精氨酸和P-His的含量升高,小脑制剂中p -精氨酸、P-His和未知化合物的含量升高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation, properties and P content of the human brain myosin.

KCl-, and NaCl-myosins were prepared from different parts of the central nervous system (CNS). Throughout these experiments P and lipid contents were higher in NaCl-myosins than in KCl-preparations. Both KCl-, and NaCl-myosins have increased lipid and P contents compared with skeletal muscle myosins. When the specimens were separated by a molecular sieve, it was found by chromatographic technique on Sepharose 4B column that the cerebral and cerebellar myosins were composed of two fractions of different molecular mass while the brain stem and spinal cord myosins revealed only a single peak. The myosin fractions' Ca-ATPase activity could be augmented by rabbit muscle actin. The myosin preparations developed filamentous systems and aggregates which could be shown by scanning electron microscopy. All the CNS-myosin preparations could be phosphorylated; however, they were saturated to a different degree and were influenced by the presence or absence of serotonin. The kinetic studies revealed that the phosphate saturation of the brain stem, cerebellar and cerebral myosins depended on the ATP concentration and incubation time. The alkaline hydrolysates of lipid-free human brain myosin preparations contained amino acid phosphates, P-Arg, P-Lys and P-His in different amounts depending on their sources. In response to a phosphorylating mixture only the amount of P-Arg was elevated in the cerebral myosins, P-Arg and P-His in the brain stem preparations, and P-Arg, P-His and the amounts of unidentified compounds in the cerebellar ones.

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