节前刺激大鼠颈上神经节诱发神经垂体后叶催产素释放。

Endokrinologie Pub Date : 1982-07-01
S Lipinska, M Orłowska-Majdak, G Rubacha, W Z Traczyk
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引用次数: 0

摘要

1. 聚氨酯/氯氯蔗糖麻醉下的雄性大鼠经咽入路暴露垂体。采用以下方法:(a)垂体后叶原位孵育,切除前叶,抬高后叶,使其与脑膜之间的血管断裂,并连续交换孵育液;(b)切除整个垂体,并连续更换冲洗垂体柄近端切口的液体。2. 根据Van Dongen和Hays(1966)的方法,收集20 - 30分钟的孵育或洗涤液样品3-5小时,并检测催产素。3.节前刺激颈上神经节导致垂体后叶释放的催产素显著增加(约25倍)。4. 节前刺激颈上神经节不改变冲洗垂体柄近端切口的液体中的催产素含量。5. 颈上神经节交感神经元能诱导脑垂体释放催产素,但不能改变其从下丘脑向脑垂体的流出。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Oxytocin release from neurohypophysis evoked by preganglionic stimulation of the rat superior cervical ganglion.

1. In male rats under urethane/chloralose anaesthesia the pituitary gland was exposed by transpharyngeal approach. The following methods were applied: (a) posterior pituitary lobe incubation in situ with the anterior lobe removed and the posterior lobe elevated so that only the vessels between the lobe and the meninges are broken, and continuous exchange of the incubation fluid; (b) removal of the entire pituitary and continuous exchange of the fluid washing the cut proximal end of the pituitary stalk. 2. 20 to 30-min samples of incubation or washing fluid were collected for 3-5 hrs and oxytocin was assayed according to Van Dongen and Hays (1966). 3. Preganglionic stimulation of the superior cervical ganglion caused significant increase (ca. 25 times) of oxytocin release from the posterior pituitary lobe. 4. Preganglionic stimulation of the superior cervical ganglion did not alter oxytocin content in the fluid washing the cut proximal end of the pituitary stalk. 5. The sympathetic neurons of the superior cervical ganglia could evoked oxytocin release from the neurohypophysis but did not change its outflow from the hypothalamus to the neurohypophysis.

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