{"title":"甘油对兔肝微粒体细胞色素P-450稳定作用的定量特征。","authors":"D I Metelitza, A N Eryomin, S A Usanov","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Kinetics of thermal inactivation of the rabbit liver microsomal cytochrome P-450 has been studied in the temperature range of 45-54 degrees C with increasing concentrations of glycerol. For all temperatures studied in the absence and presence of glycerol, thermal inactivation of cytochrome P-450 is characterized by two phases, the first being described by the first order rate constants. Glycerol markedly lowers the rate of thermal inactivation of cytochrome P-450 as well as the activation parameters delta H and delta S of the process of thermal destruction. There is a linear relationship between decreasing values of delta H, delta S and increasing glycerol concentrations in the medium.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 1","pages":"17-21"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantitative characteristics of the stabilizing effect of glycerol on cytochrome P-450 in rabbit liver microsomes.\",\"authors\":\"D I Metelitza, A N Eryomin, S A Usanov\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Kinetics of thermal inactivation of the rabbit liver microsomal cytochrome P-450 has been studied in the temperature range of 45-54 degrees C with increasing concentrations of glycerol. For all temperatures studied in the absence and presence of glycerol, thermal inactivation of cytochrome P-450 is characterized by two phases, the first being described by the first order rate constants. Glycerol markedly lowers the rate of thermal inactivation of cytochrome P-450 as well as the activation parameters delta H and delta S of the process of thermal destruction. There is a linear relationship between decreasing values of delta H, delta S and increasing glycerol concentrations in the medium.</p>\",\"PeriodicalId\":6985,\"journal\":{\"name\":\"Acta biologica et medica Germanica\",\"volume\":\"41 1\",\"pages\":\"17-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1982-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta biologica et medica Germanica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta biologica et medica Germanica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Quantitative characteristics of the stabilizing effect of glycerol on cytochrome P-450 in rabbit liver microsomes.
Kinetics of thermal inactivation of the rabbit liver microsomal cytochrome P-450 has been studied in the temperature range of 45-54 degrees C with increasing concentrations of glycerol. For all temperatures studied in the absence and presence of glycerol, thermal inactivation of cytochrome P-450 is characterized by two phases, the first being described by the first order rate constants. Glycerol markedly lowers the rate of thermal inactivation of cytochrome P-450 as well as the activation parameters delta H and delta S of the process of thermal destruction. There is a linear relationship between decreasing values of delta H, delta S and increasing glycerol concentrations in the medium.
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