[脂质脂肪酸不饱和对大肠杆菌细胞质膜结构和功能的影响]。

E J Shechter, L Y Letellier
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引用次数: 0

摘要

本文的研究结果建立了大肠杆菌不饱和脂肪营养不良菌细胞质膜囊泡的结构、形态和功能特性之间的关系。这些膜是从存在油酸、亚麻酸或油酸的细胞中分离出来的。高角度x射线衍射研究表明,温度变化引起的与脂质烃链相关的有序-无序转变是膜脂肪酸组成的函数。在某些情况下,各种脂质的“共结晶”发生在单一类型的有序结构域内。在其他情况下,存在多种脂质分离成一个以上不同类型的有序结构域。观察到的各种有序-无序转变引起了膜疏水核心的形态变化,这些变化可以通过冷冻断裂电子显微镜检测到。当脂质烃链紊乱时,观察到颗粒在裂缝面上的随机分布。在烃链排序后,颗粒被排除在有序区域之外,因此,可以观察到光滑区域和颗粒密集的区域。能量依赖性β -半乳糖苷摄取速率作为温度函数的不连续与观察到的有序-无序转变有关。在过渡的温度区域,Arrhenius表示的斜率增加与被排除在有序膜结构域之外的载体蛋白的分离以及它们随后在含有无序脂质的膜结构域的聚集有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effect of the unsaturation of lipids fatty acids on the structure and function of the cytoplasmic membranes of E. coli].

The results presented in this paper establish relationships between structural, morphological and functional properties of cytoplasmic membrane vesicles isolated from an E. coli unsaturated fatty auxotroph. The membranes were isolated from cells grown in the presence of either oleic, linolenic or elaidic acids. High-angle X-ray diffraction studies show that order-disorder transitions induced by temperature variation sand associated with the hydrocarbon chains of the lipids are a function of the fatty acid composition of the membranes. In some cases, "cocrystallization" of various lipid species takes place within a single type of ordered domains. In other cases, there is a segregation of various lipid species into more than one distinct type of ordered domain. The various order-disorder transitions observed, induce morphological changes in the hydrophobic core of the membranes which can be detected by freeze-fracture electron microscopy. A random distribution of particles on the fracture faces is observed when the hydrocarbon chains of the lipids are disordered. Upon ordering of the hydrocarbon chains, particles are excluded from the ordered domains and as a consequence, smooth areas and areas with dense ly packed particles are observed. Discontinuities in the rate of energy-dependent beta-galactoside uptake as a function of temperature correlate with the order-disorder transitions observed. The increased slope of the Arrhenius representation in the temperature region of the transition is associated with the segregation of the carrier proteins excluded from the ordered membrane domains and their subsequent aggregation in the membrane domains containing the disordered lipids.

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