雌激素对去卵巢大鼠下丘脑突触体中促黄体生成素释放激素的影响。

M Tytell, J H Clark, E J Peck
{"title":"雌激素对去卵巢大鼠下丘脑突触体中促黄体生成素释放激素的影响。","authors":"M Tytell,&nbsp;J H Clark,&nbsp;E J Peck","doi":"10.3109/07435808009065957","DOIUrl":null,"url":null,"abstract":"<p><p>Previous investigations have shown that hypothalamic luteinizing hormone releasing hormone (LHRH) is localized within a subcellular fraction eqivalent or similar to the synaptosome or nerve ending particle. In the present study, the ovariectomized adult rat was employed to investigate the effects of estrogen on the distribution of LHRH among various subcellular fractions of the hypothalamus. In addition, the liberation of LHRH from a synaptosomal fraction by repeated freeze-thaw cycles was examined before and after in vivo estrogen treatment. Following a single injection of 5 micrograms of estradiol benzoate (EB), the LHRH concentration of the synaptosomal fraction rose as much as 100% in 27 h. Increasing the dose of EB to 50 micrograms shortened the latency of the rise and prolonged its duration, but did not alter its magnitude compared to the lower dose. Furthermore, the LHRH in that synaptosomal fraction was significantly more resistant to solubilization by repetitive freezing and thawing than the LHRH in a synaptosomal fraction from rats which had not received EB. These observations led us to conclude that estrogens can alter the physical properties of the hypothalamic LHRH-containing particles or the storage form of the peptide within those particles so that it is less easily released. This phenomenon may represent one aspect of the regulatory effects of estrogens on hypothalamic LHRH secretion.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"7 1","pages":"27-44"},"PeriodicalIF":0.0000,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435808009065957","citationCount":"9","resultStr":"{\"title\":\"Effects of estrogen on luteinizing hormone releasing hormone in a hypothalamic synaptosomal fraction from the ovariectomized rat.\",\"authors\":\"M Tytell,&nbsp;J H Clark,&nbsp;E J Peck\",\"doi\":\"10.3109/07435808009065957\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Previous investigations have shown that hypothalamic luteinizing hormone releasing hormone (LHRH) is localized within a subcellular fraction eqivalent or similar to the synaptosome or nerve ending particle. In the present study, the ovariectomized adult rat was employed to investigate the effects of estrogen on the distribution of LHRH among various subcellular fractions of the hypothalamus. In addition, the liberation of LHRH from a synaptosomal fraction by repeated freeze-thaw cycles was examined before and after in vivo estrogen treatment. Following a single injection of 5 micrograms of estradiol benzoate (EB), the LHRH concentration of the synaptosomal fraction rose as much as 100% in 27 h. Increasing the dose of EB to 50 micrograms shortened the latency of the rise and prolonged its duration, but did not alter its magnitude compared to the lower dose. Furthermore, the LHRH in that synaptosomal fraction was significantly more resistant to solubilization by repetitive freezing and thawing than the LHRH in a synaptosomal fraction from rats which had not received EB. These observations led us to conclude that estrogens can alter the physical properties of the hypothalamic LHRH-containing particles or the storage form of the peptide within those particles so that it is less easily released. This phenomenon may represent one aspect of the regulatory effects of estrogens on hypothalamic LHRH secretion.</p>\",\"PeriodicalId\":75821,\"journal\":{\"name\":\"Endocrine research communications\",\"volume\":\"7 1\",\"pages\":\"27-44\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/07435808009065957\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Endocrine research communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/07435808009065957\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine research communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/07435808009065957","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9

摘要

以往的研究表明,下丘脑黄体生成素释放激素(LHRH)定位于相当于或类似于突触体或神经末节颗粒的亚细胞部分。本研究采用去卵巢的成年大鼠,研究雌激素对下丘脑各亚细胞部分LHRH分布的影响。此外,在体内雌激素治疗前后,通过反复冻融循环检测LHRH从突触体部分释放的情况。单次注射5微克苯甲酸雌二醇(estradiol benzoate, EB)后,突触体部分LHRH浓度在27小时内上升100%。将EB剂量增加到50微克可缩短上升的潜伏期并延长其持续时间,但与较低剂量相比,其幅度没有改变。此外,与未接受EB治疗的大鼠突触体片段相比,该突触体片段中的LHRH对反复冷冻和解冻的溶解作用具有更强的抵抗力。这些观察结果使我们得出结论,雌激素可以改变下丘脑含有lhrh的颗粒的物理性质或这些颗粒中肽的储存形式,从而使其不易释放。这一现象可能代表了雌激素对下丘脑LHRH分泌调节作用的一个方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of estrogen on luteinizing hormone releasing hormone in a hypothalamic synaptosomal fraction from the ovariectomized rat.

Previous investigations have shown that hypothalamic luteinizing hormone releasing hormone (LHRH) is localized within a subcellular fraction eqivalent or similar to the synaptosome or nerve ending particle. In the present study, the ovariectomized adult rat was employed to investigate the effects of estrogen on the distribution of LHRH among various subcellular fractions of the hypothalamus. In addition, the liberation of LHRH from a synaptosomal fraction by repeated freeze-thaw cycles was examined before and after in vivo estrogen treatment. Following a single injection of 5 micrograms of estradiol benzoate (EB), the LHRH concentration of the synaptosomal fraction rose as much as 100% in 27 h. Increasing the dose of EB to 50 micrograms shortened the latency of the rise and prolonged its duration, but did not alter its magnitude compared to the lower dose. Furthermore, the LHRH in that synaptosomal fraction was significantly more resistant to solubilization by repetitive freezing and thawing than the LHRH in a synaptosomal fraction from rats which had not received EB. These observations led us to conclude that estrogens can alter the physical properties of the hypothalamic LHRH-containing particles or the storage form of the peptide within those particles so that it is less easily released. This phenomenon may represent one aspect of the regulatory effects of estrogens on hypothalamic LHRH secretion.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信