胎牛血清对E型玫瑰花试验结果的影响。

A Naess
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引用次数: 0

摘要

绵羊红细胞(E)花环试验在培养基中添加和不添加胎牛血清(FCS),并使用未经处理和2-氨基乙基硫代异ourium bromide - hydrobromide (AET)处理的E。34名正常供者外周血淋巴细胞在添加或不添加FCS的试验中,E花环百分比无显著差异。然而,在培养基中使用未经处理的含有FCS的E时,传染性单核细胞增多症患者和无菌性脑膜炎患者的玫瑰花形成细胞(RFC)百分比明显低于不含FCS的患者。当使用aet治疗的E时,加入FCS增加了传染性单核细胞增多症、稳定型多发性硬化症和脑肿瘤患者淋巴细胞的玫瑰花结形成。使用FCS时,经aet治疗的E的莲座形成通常增加,而当FCS与未治疗的E联合使用时,莲座形成减少更为常见。事实上,在某些患者组中发现了与FCS相关的莲座形成的显着差异,这要求在比较不同使用或不使用FCS的实验室结果时要谨慎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of fetal calf serum on the results of the E rosette test.

The sheep erythrocyte (E) rosette test was performed with and without fetal calf serum (FCS) in the medium, and using untreated and 2-amino-ethylthioisouronium bromide hydrobromide (AET)-treated E. Peripheral blood lymphocytes from 34 normal donors showed no significant difference in E rosette percentage between the tests performed with or without FCS. However, when using untreated E with FCS in the medium, patients with infectious mononucleosis and those with aseptic meningitis had a significantly decreased percentage of rosette-forming cells (RFC) as compared to the test performed without FCS. When AET-treated E were used, addition of FCS increased rosette formation of lymphocytes from patients with infectious mononucleosis, with stable multiple sclerosis and with brain tumours. Rosette formation with AET-treated E was generally increased when FCS was used, whereas decreased rosette formation was more common when FCS was used in combination with untreated E. The fact that significant differences in rosette formation associated with FCS have been found in certain patient groups, calls for caution when comparing results from laboratories that differ in the use or not of FCS.

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