脂肪酸组成对大肠杆菌膜功能对乙醇敏感性的影响。

Substance and alcohol actions/misuse Pub Date : 1982-01-01
L C Eaton, T F Tedder, L O Ingram
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引用次数: 0

摘要

研究了大肠杆菌中膜组成对膜功能对乙醇敏感性的影响。体外添加乙醇(0.67M)对NADH氧化酶、d -乳酸氧化酶和atp酶没有明显的抑制作用,但对琥珀酸脱氢酶、谷氨酸摄取、脯氨酸摄取和1ac渗透酶有11% ~ 30%的抑制作用。虽然这些膜功能对乙醇的敏感性随膜成分的不同而变化,但没有一个与乙醇杀伤敏感性的变化相关。相比之下,亮氨酸在对照细胞和富含异丙酸的细胞中对乙醇(0.67M)具有抗性,但在棕榈酸中生长的细胞中被抑制了25%。核苷酸的释放被检测为细胞通透性的比较测量。乙醇增加核苷酸泄漏。在异丙酸中生长的细胞中渗漏减少,而在富含棕榈酸的细胞中渗漏增加。MgSO4 (10mM)的加入减少了核苷酸泄漏,提高了存活率。基于这些结果,代谢物泄漏被认为是与乙醇缓冲溶液中细菌失活相关的主要事件。酰基链长度的增加被认为是异丙酸掺入的有益方面,而不是膜不饱和的增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of fatty acid composition on the sensitivity of membrane functions to ethanol in Escherichia coli.

The effects of membrane composition on the sensitivity of membrane functions to ethanol in Escherichia coli have been investigated. The addition of ethanol (0.67M) in vitro did not cause appreciable inhibition of NADH oxidase, D-lactate oxidase or ATPase but caused an 11% to 30% inhibition of succinic dehydrogenase, glutamate uptake, proline uptake and 1ac permease. Although the sensitivities of some of these membrane functions to ethanol varied with membrane composition, none correlated with the changes in sensitivity to killing by ethanol. In contrast, leucine transport was resistant to ethanol (0.67M) in control cells and in cells enriched in vaccenic acid, but was inhibited by 25% in cells grown in palmitic acid. The release of nucleotides was examined as a comparative measure of cellular permeability. Ethanol increased nucleotide leakage. Leakage was reduced in cells grown in vaccenic acid and enhanced in cells enriched in palmitic acid. The addition of MgSO4 (10mM) reduced nucleotide leakage and enhanced survival. Based upon these results, metabolite leakage was proposed as the primary event associated with bacterial inactivation in buffered solutions of ethanol. The increase in acyl chain length is proposed as the beneficial aspect of vaccenic acid incorporation rather than the increase in membrane unsaturation.

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