{"title":"高能辐射诱导组蛋白八聚体复合物的交联。","authors":"K J Deeg, L Katsikas, W Schnabel","doi":"10.1515/znc-1984-11-1213","DOIUrl":null,"url":null,"abstract":"<p><p>Calf thymus histone octamer complexes were irradiated in the native state in N2O-saturated dilute aqueous solution (0.5 g/l, pH 9, [NaClO4] = 1 -4 mol/l) with 50 or 100 ns pulses of 16 MeV electrons or 60Co-gamma-rays. Time resolved light scattering measurements and optical absorption measurements yielded the following: the octamers underwent a volume contraction due to intra-complex-crosslinking induced by the attack of OH-radicals. Crosslinking proceeded to a certain extent via 2,2'-biphenol coupling as inferred from product analyses.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1075-8"},"PeriodicalIF":0.0000,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-11-1213","citationCount":"3","resultStr":"{\"title\":\"High energy radiation-induced crosslinking of histone octamer complexes.\",\"authors\":\"K J Deeg, L Katsikas, W Schnabel\",\"doi\":\"10.1515/znc-1984-11-1213\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Calf thymus histone octamer complexes were irradiated in the native state in N2O-saturated dilute aqueous solution (0.5 g/l, pH 9, [NaClO4] = 1 -4 mol/l) with 50 or 100 ns pulses of 16 MeV electrons or 60Co-gamma-rays. Time resolved light scattering measurements and optical absorption measurements yielded the following: the octamers underwent a volume contraction due to intra-complex-crosslinking induced by the attack of OH-radicals. Crosslinking proceeded to a certain extent via 2,2'-biphenol coupling as inferred from product analyses.</p>\",\"PeriodicalId\":23914,\"journal\":{\"name\":\"Zeitschrift fur Naturforschung. Section C, Biosciences\",\"volume\":\"39 11-12\",\"pages\":\"1075-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/znc-1984-11-1213\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur Naturforschung. Section C, Biosciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/znc-1984-11-1213\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Naturforschung. Section C, Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/znc-1984-11-1213","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
High energy radiation-induced crosslinking of histone octamer complexes.
Calf thymus histone octamer complexes were irradiated in the native state in N2O-saturated dilute aqueous solution (0.5 g/l, pH 9, [NaClO4] = 1 -4 mol/l) with 50 or 100 ns pulses of 16 MeV electrons or 60Co-gamma-rays. Time resolved light scattering measurements and optical absorption measurements yielded the following: the octamers underwent a volume contraction due to intra-complex-crosslinking induced by the attack of OH-radicals. Crosslinking proceeded to a certain extent via 2,2'-biphenol coupling as inferred from product analyses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
