一种从猪白细胞大规模无血清培养物中部分纯化的颗粒生成抑制剂。

M Kastner, H R Maurer, U Gerlach, H Renner, J H Wissler
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引用次数: 2

摘要

本发明描述了一种从相当于1kg或2 × 10(12)个猪血液白细胞的200 l批中提取的肉芽肿生成抑制剂的部分纯化方法。无血清培养基通过硫酸铵沉淀、丙酮分配、超滤和凝胶层析进行浓缩分离。活性物质的分子质量为500-700 Da,不依赖硫醇,因此与先前报道的颗粒生成抑制剂形成对比。在体外骨髓干细胞实验中,使用各种集落刺激因子抑制集落形成表明成熟的粒细胞和单核细胞同样受到影响。用一种新的可逆性试验表明明显缺乏细胞毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A granulopoiesis inhibitor partially purified from large-scale serum-free cultures of porcine leukocytes.

A method is described for the partial purification of a granulomonopoiesis inhibitor derived from 200 l-batches equivalent to 1 kg or 2 X 10(12) leukocytes of porcine blood. Serum-free conditioned media were concentrated and separated via ammonium sulfate precipitation, acetone partitioning, ultrafiltration and gel chromatography. The active substance revealed a molecular mass of 500-700 Da and was not thiol-dependent, thus contrasting it with previously-reported granulopoiesis inhibitors. Inhibition of colony formation in an in vitro myeloid stem cell assay using various colony-stimulating factors showed that mature granulocytes and monocytes were equally affected. Apparent lack of cytotoxicity was suggested using a new reversibility test.

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