[从天然底物中定向分离革兰氏阴性菌种]。

Antibiotiki Pub Date : 1984-10-01

L P Ivanitskaia, E M Singal, L E Bodunkova, L N Ostanina, M V Baturina

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引用次数: 0

摘要

建立了一种从土壤基质中选择性分离革兰氏阴性无孢子菌的方法。该方法包括将底物镀在葡萄糖-酵母培养基上，并添加青霉素和萘啶酸。革兰氏阴性非孢子菌的分离率从对照的9- 16%提高到80- 100%，革兰氏阳性菌的分离率从88.5%下降到9.6%。

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[Directed isolation of gram-negative asporogenous bacteria from natural substrates].

A method for selective isolation of gramnegative nonsporulating bacteria from soil substrates was developed. The method consists of plating out the substrates on a glucose-yeast medium with addition of benzylpenicillin and nalidixic acid. The isolation frequency of gramnegative nonsporulating bacteria increased under such conditions from 9-16 (control) to 80-100 per cent. At the same time the isolation frequency of gram-positive bacteria decreased from 88.5 to 9.6 per cent.

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