{"title":"酶联免疫吸附法与放射免疫法测定抗肺炎球菌多糖抗体的比较。","authors":"F K Pedersen, J Henrichsen, G Schiffman","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Antibodies against pneumococcal capsular polysaccharide types 1, 3, 6A, 7F, 8 and 9N were determined before and 4 weeks after pneumococcal vaccination by both enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) in 49 children, 42 of whom had been splenectomized. Using linear regression analysis a significant correlation between total antibody concentrations determined by ELISA and RIA was found for all 6 antigens (p less than 0.001 for each). The degree of correlation varied between types, antibodies against type 3 showing the best concordance, those against type 6A the poorest. In 84% of the samples assayed both the RIA and the ELISA determined antibody concentrations were either above (55%) or below (29%) the hypothetical protective antibody concentration.</p>","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 4","pages":"251-5"},"PeriodicalIF":0.0000,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of enzyme-linked immunosorbent assay and radioimmunoassay for determination of anti-pneumococcal polysaccharide antibodies.\",\"authors\":\"F K Pedersen, J Henrichsen, G Schiffman\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Antibodies against pneumococcal capsular polysaccharide types 1, 3, 6A, 7F, 8 and 9N were determined before and 4 weeks after pneumococcal vaccination by both enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) in 49 children, 42 of whom had been splenectomized. Using linear regression analysis a significant correlation between total antibody concentrations determined by ELISA and RIA was found for all 6 antigens (p less than 0.001 for each). The degree of correlation varied between types, antibodies against type 3 showing the best concordance, those against type 6A the poorest. In 84% of the samples assayed both the RIA and the ELISA determined antibody concentrations were either above (55%) or below (29%) the hypothetical protective antibody concentration.</p>\",\"PeriodicalId\":77653,\"journal\":{\"name\":\"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology\",\"volume\":\"91 4\",\"pages\":\"251-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of enzyme-linked immunosorbent assay and radioimmunoassay for determination of anti-pneumococcal polysaccharide antibodies.
Antibodies against pneumococcal capsular polysaccharide types 1, 3, 6A, 7F, 8 and 9N were determined before and 4 weeks after pneumococcal vaccination by both enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) in 49 children, 42 of whom had been splenectomized. Using linear regression analysis a significant correlation between total antibody concentrations determined by ELISA and RIA was found for all 6 antigens (p less than 0.001 for each). The degree of correlation varied between types, antibodies against type 3 showing the best concordance, those against type 6A the poorest. In 84% of the samples assayed both the RIA and the ELISA determined antibody concentrations were either above (55%) or below (29%) the hypothetical protective antibody concentration.
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