Edmond E. Creppy , Maryvonne Mayer , Daniel Kern , Monique Schlegel , Pieter S. Steyn , Robert Vleggaar , Guy Dirheimer
{"title":"赭曲霉毒素A缬氨酸同源物对酵母缬氨酸- trna合成酶的体外抑制作用","authors":"Edmond E. Creppy , Maryvonne Mayer , Daniel Kern , Monique Schlegel , Pieter S. Steyn , Robert Vleggaar , Guy Dirheimer","doi":"10.1016/0005-2787(81)90095-2","DOIUrl":null,"url":null,"abstract":"<div><p>An analogue of valine in which valine is coupled by its amino group to a chlorinated dihydroisocoumarin moiety inhibits competitively the tRNA<sup>Val</sup> charging with valine catalyzed by pure yeast valyl-tRNA synthetase. The inhibition constant is much lower for the ATPaiPP<sub>i</sub> exchange reaction than for the tRNA charging one, indicating different rate-determining steps in the two reactions.</p></div>","PeriodicalId":100164,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","volume":"656 2","pages":"Pages 265-268"},"PeriodicalIF":0.0000,"publicationDate":"1981-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2787(81)90095-2","citationCount":"11","resultStr":"{\"title\":\"In vitro inhibition of yeast valyl-tRNA synthetase by the valine homologue of ochratoxin A\",\"authors\":\"Edmond E. Creppy , Maryvonne Mayer , Daniel Kern , Monique Schlegel , Pieter S. Steyn , Robert Vleggaar , Guy Dirheimer\",\"doi\":\"10.1016/0005-2787(81)90095-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An analogue of valine in which valine is coupled by its amino group to a chlorinated dihydroisocoumarin moiety inhibits competitively the tRNA<sup>Val</sup> charging with valine catalyzed by pure yeast valyl-tRNA synthetase. The inhibition constant is much lower for the ATPaiPP<sub>i</sub> exchange reaction than for the tRNA charging one, indicating different rate-determining steps in the two reactions.</p></div>\",\"PeriodicalId\":100164,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis\",\"volume\":\"656 2\",\"pages\":\"Pages 265-268\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-12-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2787(81)90095-2\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005278781900952\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005278781900952","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vitro inhibition of yeast valyl-tRNA synthetase by the valine homologue of ochratoxin A
An analogue of valine in which valine is coupled by its amino group to a chlorinated dihydroisocoumarin moiety inhibits competitively the tRNAVal charging with valine catalyzed by pure yeast valyl-tRNA synthetase. The inhibition constant is much lower for the ATPaiPPi exchange reaction than for the tRNA charging one, indicating different rate-determining steps in the two reactions.
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