内源性TSH对大鼠甲状腺组织体外反应性和TSH结合的影响。

S D Holmes, J B Field
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引用次数: 2

摘要

这些研究考察了内源性TSH缺乏(垂体切除术)对体外TSH反应性和大鼠甲状腺组织结合的影响。结果表明,去垂体大鼠甲状腺对TSH刺激环AMP反应强烈,而去垂体大鼠对环AMP的PGE1刺激与对照组相似。当测量碘化物组织时,垂体切除大鼠的TSH和二丁基环AMP刺激与对照动物的反应相当。结合研究表明,与对照组相比,垂体切除大鼠甲状腺中TSH受体的数量有所增加。亲和常数不变。结果表明,内源性TSH似乎反向调节甲状腺组织中TSH受体的反应性和数量。还研究了静脉注射TSH对环AMP水平和TSH结合特性的影响。虽然注射TSH的大鼠脑叶体外孵育10分钟后,环AMP水平显著升高,表明TSH持续结合,但注射TSH的动物生理盐水中TSH结合位点的数量相同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of endogenous TSH on the in vitro responsiveness and binding of TSH in rat thyroid tissue.

These studies examined the effect of deficient endogenous TSH (hypophysectomy) on in vitro TSH responsiveness and binding in rat thyroid tissue. The results showed that thyroid from hypophysectomized rats were hyper-responsive to stimulation of cyclic AMP by TSH while PGE1 stimulation of cyclic AMP was similar in hypophysectomized rats compared to controls. When iodide organification was measured, the TSH and dibutyryl cyclic AMP stimulation in hypophysectomized rats was equivalent to the response of control animals. Binding studies demonstrated there was an increased number of TSH receptors in the thyroid from hypophysectomized rats compared to the control group. The affinity constant was unchanged. The results demonstrate that endogenous TSH appears to inversely regulate the responsiveness and number of TSH receptors in thyroid tissue. The effect of an i.v. injection of TSH on the levels of cyclic AMP and binding characteristics of TSH was also examined. Although cyclic AMP levels were significantly increased after a 10 min in vitro incubation of the lobes from TSH-injected rats as an indication of persistently bound TSH, the number of TSH binding sites was the same in the saline the TSH-injected animals.

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