钙依赖性对硝基苯基磷酸水解肌浆网钙转运酶的活化体积。

K G König, W Hasselbach
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引用次数: 6

摘要

在温度、溶质、底物和离子浓度等不同条件下,研究了压力对肌浆网钙转运酶钙依赖性水解对硝基苯基磷酸的影响。钙转运酶表现出较大的正激活体积,这既不依赖于高盐浓度对酶的抑制,也不依赖于乙二醇对酶的激活。活化体积进一步证明与压力无关,但表现出明显的负温度系数。对硝基苯基磷酸盐、钙离子或镁离子进入底物离子络合物所引起的体积变化非常小,这表明与这些配体结合有关的水的转移被蛋白质的体积变化所补偿,伴随着酶从活化状态向基态的转变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Activation volumes of the calcium dependent para-nitrophenyl phosphate hydrolysis of the sarcoplasmic reticulum calcium transport enzyme.

The effect of pressure on the calcium dependent hydrolysis of para-nitrophenyl phosphate by the calcium transport enzyme of the sarcoplasmic reticulum was studied under different conditions: temperature, solutes, substrate and ion concentrations. The calcium transport enzyme exhibits a large positive activation volume which does neither depend on the enzyme's inhibition by high salt concentrations nor its activation by ethylene glycol. The activation volume further proves to be pressure-independent but exhibits a pronounced negative temperature coefficient. The volume changes connected with the entrance of para-nitrophenyl phosphate, calcium or magnesium ions into the substrate ion complex are quite small, indicating that the transfer of water connected with the binding of these ligands is compensated by volume changes of the protein, accompanying the transition of the enzyme from its activated into its ground state.

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